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| Status |
Public on Sep 10, 2012 |
| Title |
A gene expression signature of PDGF-stimulated fibroblasts |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Analysis of platelet-derived growth factor (PDGF)-stimulated fibroblasts. Results provide insight into novel pro-tumorigenic factors induced by PDGF-activation of fibroblasts.
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| Overall design |
1.2 x 10e6 BJ-hTERT fibroblasts were cultured in 10cm dish with Dulbecco's Modified Eagle Medium (DMEM; Gibco Life Technologies, Gergy-Pontoise, France) containing 1% heat-inactivated fetal calf serum (FCS), 2 mM L-glutamine, penicillin (100 units/ml) and streptomycin (100 ng/ml) at 37ºC in a 5% CO2 humidified atmosphere during 24h. Cultures were stimulated with or without 20ng/mL PDGF-BB (Peprotech, New Jersey, USA) for another 24h prior to harvest. Unstimulated fibroblasts were used as control samples. Cultures of both unstimulated and stimulated fibroblasts were done in triplicate.
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| Contributor(s) |
Peña C |
| Citation(s) |
23243022, 30250018 |
| Submission date |
Sep 09, 2012 |
| Last update date |
May 17, 2019 |
| Contact name |
Cristina Peña |
| E-mail(s) |
cpena@idiphim.org
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| Organization name |
Hospital Universitario Puerta de Hierro, Majadahonda
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| Street address |
Joaquín Rodrigo,2
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| City |
Majadahonda |
| State/province |
Madrid |
| ZIP/Postal code |
28222 |
| Country |
Spain |
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| Platforms (1) |
| GPL6947 |
Illumina HumanHT-12 V3.0 expression beadchip |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA174750 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE40720_RAW.tar |
6.2 Mb |
(http)(custom) |
TAR |
| GSE40720_non-normalized.txt.gz |
2.1 Mb |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
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