|
| Status |
Public on Aug 03, 2022 |
| Title |
CD4 cells re-educate tumor associated macrophages. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Tumor Associated Macrophages (TAMs) are known to release cytokines/chemokines that promote tumor survival and angiogenesis. Little is known, however, about their ability to present tumor antigens to CD4 T cells, nor whether such presentation might switch their tumor supporting function. Here we show that TAMs present tumor antigens to CD4 T cells and that upon antigen recognition, the T cells re-educate the TAMs to produce lower amounts of tumor nurturing proteins and greater amounts of anti-angiogenic proteins. The CD4-educated-TAMs display a gene signature characterized by upregulation of INFgamma esponsive genes that have anti-viral and anti-bacterial function. This phenotypic switch is INF-gamma dependent, and necessary for CD4-mediated tumor rejection. CD4-based immunotherapies could take advantage of this re-education process to improve cancer treatment.
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|
| Overall design |
The experiment consisted of six untreated Tumor Associated Macrophages (TAMs) and six CD4-treated Tumor Associated Macrophages (TAMs) for a total of 12 samples. For each of the treated and untreated samples, two were technical replicates and four were biological replicates.
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| |
|
| Citation(s) |
35903540 |
| Submission date |
Sep 17, 2012 |
| Last update date |
Aug 04, 2022 |
| Contact name |
Ainhoa Perez-Diez |
| E-mail(s) |
ainhoa@nih.gov
|
| Phone |
(301) 496-3503
|
| Organization name |
NIAID, NIH
|
| Lab |
Lab of Immunoregulation
|
| Street address |
9000 Rockville Pike
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL6887 |
Illumina MouseWG-6 v2.0 expression beadchip |
|
| Samples (12)
|
| GSM1005101 |
CD4-Treated TAMs - technical repeat 1 - mAdbID:116689 |
| GSM1005102 |
Untreated TAMs - biological repeat 1 - mAdbID:116690 |
| GSM1005103 |
CD4-Treated TAMs - biological repeat 2 - mAdbID:116691 |
| GSM1005104 |
Untreated TAMs - biological repeat 2 - mAdbID:116692 |
| GSM1005105 |
CD4-Treated TAMs - technical repeat 3 - mAdbID:116693 |
| GSM1005106 |
Untreated TAMs - technical repeat 3 - mAdbID:116694 |
| GSM1005107 |
Untreated TAMs - biological repeat 4 - mAdbID:116695 |
| GSM1005108 |
CD4-Treated TAMs - biological repeat 4 - mAdbID:116696 |
| GSM1005109 |
CD4-Treated TAMs - biological repeat 5 - mAdbID:116697 |
| GSM1005110 |
Untreated TAMs - technical repeat 5 - mAdbID:116698 |
| GSM1005111 |
CD4-Treated TAMs - biological repeat 6 - mAdbID:116699 |
| GSM1005112 |
Untreated TAMs - biological repeat 6 - mAdbID:116700 |
|
| Relations |
| BioProject |
PRJNA175464 |
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