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| Status |
Public on Sep 21, 2012 |
| Title |
Transcriptional profiling of hypoxic cells and identification of HEXIM1-dependent genes. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
To identify primary response genes (PRGs) that were differently expressed within 1 hr of hypoxia exposure, we performed global transcriptional profiling using the Agilent Technologies SurePrint G3 Human GE Microarray .
To identify Hexim1-dependent genes, we compare profiles of hypoxia PRGs in cells transfected with control or Hexim1 targeting double-strand siRNA
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| Overall design |
HeLa cells were transfected with control and HEXIM1-targeted double-strand siRNA and 48 hr after treated with IL-1beta in combination with normoxia or hypoxia (5% CO2, 0.5% O2, balanced with N2) O for 1 hr.
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| |
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| Contributor(s) |
Safronova OS, Morita I |
| Citation(s) |
25056306 |
| Submission date |
Sep 20, 2012 |
| Last update date |
Oct 11, 2016 |
| Contact name |
Olga Sergeevna Safronova |
| E-mail(s) |
olga.cell@tmd.ac.jp
|
| Phone |
81-3-5803-5577
|
| Fax |
81-3-5803-0212
|
| Organization name |
Tokyo Medical and Dental University
|
| Department |
Cellular Physiological Chemistry
|
| Street address |
Bunkyo-ku, Yushima, 1-5-45
|
| City |
Tokyo |
| ZIP/Postal code |
113-8549 |
| Country |
Japan |
| |
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| Platforms (1) |
| GPL14550 |
Agilent-028004 SurePrint G3 Human GE 8x60K Microarray (Probe Name Version) |
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| Samples (5)
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| Relations |
| BioProject |
PRJNA175557 |
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