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| Status |
Public on Nov 26, 2012 |
| Title |
Genome-wide TNFα-induced p65 binding before and after telomerase inhibition in HeLa cells |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Chromatin immunoprecipitation sequencing (ChIP-seq) was performed to analyze the effect of telomerase inhibition on TNFα-induced genome-wide p65 binding in HeLa cells. By obtaining over 40 million uniquely mappable reads per sample from ChIP-seq, maps for TNFα-induced p65 binding in absence and presence of an hTERT inhibitor, MST-312, were generated. As expected, TNFα treatment significantly increased genome-wide p65 occupancy. Interestingly, when cells were treated with MST-312 prior to TNFα stimulation, the number of p65 binding sites was reduced. In addition, some binding sites, including important p65 targets like IL6 and TNF, showed a reduced p65 occupancy with a minimum fold change of 1.5, after MST-312 exposure. Taken together, our ChIP-seq data indicate that telomerase is required for optimal p65 binding at a small proportion of p65 target sites upon inflammatory stimuli.
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| Overall design |
Examination of p65 binding in HeLa cells in absence and presence of TNFα and MST-312.
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| Contributor(s) |
Saginc G, Zhang Z, Li G, Ghosh A, Leow SC, Liu ET, Tergaonkar V |
| Citation(s) |
23159929 |
| Submission date |
Sep 24, 2012 |
| Last update date |
May 15, 2019 |
| Contact name |
Gaye Saginc |
| E-mail(s) |
gayesaginc@gmail.com
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| Organization name |
A*STAR
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| Department |
Genome Institute of Singapore
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| Lab |
Cancer Biology
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| Street address |
60 Biopolis Street
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| City |
Singapore |
| ZIP/Postal code |
138672 |
| Country |
Singapore |
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| Platforms (1) |
| GPL13393 |
AB SOLiD 4 System (Homo sapiens) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA175811 |
| SRA |
SRP015860 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE41100_RAW.tar |
686.1 Mb |
(http)(custom) |
TAR (of BED) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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