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| Status |
Public on Sep 17, 2013 |
| Title |
Expression data for HT29 cells treated with 5-aza-deoxy-cytidine [Affymetrix] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
The RNA samples from HT-29 (ATCC) colon cancer cell line were reverse transcribed into cDNAs and categorized in 3 groups with different concentrations of 5-aza-deoxy-cytidine (5-Aza); in each group three replicative 150 mm cultures were treated with: 1) dimethyl sulfoxide (vehicle alone, 0 μM 5-Aza); 2) 5μM 5-Aza and 3) 10 μM 5-Aza; for five days
We then used Affymetrix microarray platform to profile the gene expression of the 3 HT29 cell groups (3 replicates in each group) in order to search for differentially expressed genes
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| Overall design |
The transcriptional response of HT-29 (ATCC) colon cancer cell line under 3 concentrations of 5-aza-deoxy-cytidine was investigated based on their RNA expression profiles
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| Contributor(s) |
Li E, Williams J, McCombie WR, Denoya P, Xu X |
| Citation(s) |
23902433 |
| Submission date |
Oct 05, 2012 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Xiao Xu |
| E-mail(s) |
slowsmile820@gmail.com
|
| Organization name |
Stony Brook University BME
|
| Street address |
Stony Brook University
|
| City |
Stony Brook |
| State/province |
NY |
| ZIP/Postal code |
11794 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (9)
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| GSM1015544 |
HT29 at 5 μM of 5-Aza, biological rep1 |
| GSM1015545 |
HT29 at 5 μM of 5-Aza, biological rep2 |
| GSM1015546 |
HT29 at 5 μM of 5-Aza, biological rep3 |
| GSM1015547 |
HT29 at 10 μM of 5-Aza, biological rep1 |
| GSM1015548 |
HT29 at 10 μM of 5-Aza, biological rep2 |
| GSM1015549 |
HT29 at 10 μM of 5-Aza, biological rep3 |
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| This SubSeries is part of SuperSeries: |
| GSE41588 |
Expression data for HT29 cells treated with 5-aza-deoxy-cytidine |
|
| Relations |
| BioProject |
PRJNA176692 |
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