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| Status |
Public on Dec 12, 2012 |
| Title |
Whole heart transcriptional response to complete and epiblast-specfic deletion of Zic3 in 15.5 dpc mouse embryos |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
In order to better understand the molecular basis for the heart defects seen in Zic3 null and epiblast CKO embryos, we investigated whether complete or epiblast-specific deletion of Zic3 would impact later embryonic heart development at the transcriptional level by whole genome expression microarray.
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| Overall design |
The whole heart was carefully dissected out from 15.5 dpc Zic3 +/y, Zic3 flox/y, Zic3 flox/y; Sox2-cre, and Zic3 -/y embryos, total RNAs were extracted and purified using RNeasy Mini Kit (QIAGEN). Spectrophotometry (NanoDrop-1000 Spectrophotometer, Thermo Fisher Scientific) and microfluidic electrophoresis (Experion Automated Electrophoresis System, Bio-Rad Laboratories) were used for RNA quality control. In vitro transcription was performed using Illumina TotalPrep RNA Amplification Kit (Applied Biosystems/Ambion). cRNAs were hybridized onto Illumina MouseWG-6 v2.0 Expression BeadChips (Illumina) per manufacturer’s instructions.
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| Contributor(s) |
Jiang Z, Belmont JW |
| Citation(s) |
23184148 |
| Submission date |
Oct 17, 2012 |
| Last update date |
Jan 16, 2019 |
| Contact name |
Zhengxin Jiang |
| Organization name |
BAYLOR COLLEGE OF MEDICINE
|
| Department |
MOLECULAR AND HUMAN GENETICS
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| Lab |
JOHN BELMONT LAB
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| Street address |
ONE BAYLOR PLAZA
|
| City |
HOUSTON |
| State/province |
Texas |
| ZIP/Postal code |
77030 |
| Country |
USA |
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| Platforms (1) |
| GPL6887 |
Illumina MouseWG-6 v2.0 expression beadchip |
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| Samples (22)
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| Relations |
| BioProject |
PRJNA177872 |
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