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| Status |
Public on Oct 25, 2012 |
| Title |
Dynamic Distribution of Linker Histone H1.5 in Cellular Differentiation |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Linker histones are essential components of chromatin but the distributions and functions of many during cellular differentiation is not well understood. Here, we show that H1.5 binds to genic and intergenic regions, forming blocks of enrichment, in differentiated human cells from all three embryonic germ layers but not in embryonic stem cells. In differentiated cells, H1.5, but not H1.3, binds preferentially to genes that encode membrane and membrane-related proteins. Strikingly, 37% of H1.5 target genes belong to gene family clusters, groups of homologous genes that are located in proximity to each other on chromosomes. H1.5 binding is associated with gene repression and is required for SIRT1 binding, H3K9me2 enrichment and chromatin compaction. Depletion of H1.5 results in loss of SIRT1 and H3K9me2, increased chromatin accessibility, deregulation of gene expression and decreased cell growth. Our data reveal for the first time a specific and novel function for linker histone subtype H1.5 in maintenance of condensed chromatin at defined gene families in differentiated human cells.
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| Overall design |
Examine mRNA expression in control and H1.5 knockdown IMR90 cells
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| Contributor(s) |
Li J, Patterson M, Mikkola HK, Lowry WE, Kurdistani SK |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Oct 22, 2012 |
| Last update date |
May 15, 2019 |
| Contact name |
Jing-Yu Li |
| E-mail(s) |
jingyuli@mednet.ucla.edu
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| Organization name |
University of California, Los Angeles
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| Street address |
615 Charles E. Young Dr. South BSRB 357
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| City |
Los Angeles |
| ZIP/Postal code |
90095 |
| Country |
USA |
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| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA178224 |
| SRA |
SRP016790 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE41761_RAW.tar |
550.0 Kb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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