 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Jan 01, 2013 |
| Title |
The impact of cell source, culture methodology, culture location and individual donors on gene expression profiles of bone marrow-derived and adipose-derived stromal cells. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Gene expression was influenced most by the tissue source, followed by culture methodology, next by location where the cells were cultured and lastly the donor variability.
|
| |
|
| Overall design |
On the normalized log2 expression values per gene a multifactorial Analyses of Variance (ANOVA) was conducted. Factors CELL CULTURES (cell source), MEDIUM (culture methodology), LOCATION (culture location), and SCAN DATE (hybridization batch) were included in the ANOVA model as independent factors. The following pairwise comparisons were made; Fetal calf serum vs. Human platelet lysate, Bone marrow vs. Adipose tissue, Utrecht vs. Leiden, Utrecht vs Nijmegen, and Leiden vs Nijmegen. Changes ≥2 and Benjamini Hochberg corrected p-values <0.05 were considered significant.
|
| |
|
| Contributor(s) |
Torensma R, Prins H, Schrama E, Verwiel ET, Martens AC, Roelofs H, Jansen BJ |
| Citation(s) |
23145933 |
| |
| Submission date |
Nov 13, 2012 |
| Last update date |
Feb 18, 2019 |
| Contact name |
Ruurd Torensma |
| E-mail(s) |
r.torensma@ncmls.ru.nl
|
| Phone |
+31 24 3610544
|
| Fax |
+31 24 3540339
|
| Organization name |
Radboud University Nijmegen Medical Centre
|
| Department |
Tumorimmunology
|
| Street address |
GeertGrooteplein 28
|
| City |
Nijmegen |
| ZIP/Postal code |
6525GA |
| Country |
Netherlands |
| |
|
| Platforms (1) |
| GPL5175 |
[HuEx-1_0-st] Affymetrix Human Exon 1.0 ST Array [transcript (gene) version] |
|
| Samples (60)
|
|
| Relations |
| BioProject |
PRJNA179401 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE42245_RAW.tar |
1.4 Gb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
|
|
|
|
 |
Less...
More...