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| Status |
Public on Dec 10, 2012 |
| Title |
Transcriptional Reprogramming of Mature CD4 T helper Cells generates distinct MHC class II restricted Cytotoxic T Lymphocytes |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
We show here that the T helper-fate is not fixed and that mature antigen-stimulated CD4 T cells can switch off Thpok expression and reactivate CD8- lineage genes. This unexpected plasticity results in the post-thymic termination of the T helper- program and the functional differentiation of distinct MHC class II restricted CD4 cytotoxic T lymphocytes
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| Overall design |
Small intestine CD4 intraepithelial T lymphocytes from ThPOK-GFP reposter mice were isolated and sorted (FACS Aria) based on ThPOK and CD8aa expression. Cell were isolated either from non-experimental ThPOK-GFP reporter mice (WT) or after transfering CD4 naive T cells from ThPOK-GFP reporter mice to RAG-/-recipient animals (TR) as an experimental colitis model. Experimet was done in duplicate.
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| Contributor(s) |
Mucida D, Reis BS |
| Citation(s) |
23334788 |
| Submission date |
Nov 14, 2012 |
| Last update date |
Jun 14, 2018 |
| Contact name |
Bernardo Reis |
| E-mail(s) |
breis@rockefeller.edu
|
| Organization name |
Rockefeller University
|
| Street address |
1234 York Avenue
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10065 |
| Country |
USA |
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| Platforms (1) |
| GPL6885 |
Illumina MouseRef-8 v2.0 expression beadchip |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA179517 |
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