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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Mar 30, 2014 |
| Title |
Determination of genes affected by HERVH depletion |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
The unique cellular state of embryonic stem cells is maintained by pluripotency-associated transcription factors such as OCT4. The transcriptional regulatory circuitries between human and mouse pluripotent stem cells exhibit notable differences. Transposable elements have altered the pluripotency network by contributing new cis-regulatory elements to mammalian genomes. In our study, we found that the expression of HERVH is enriched in human embryonic stem cells (hESCs) and depletion of HERVH in hESCs induced differentiation. To confirm the differentiation phenotype and identify potential downstream target genes of HERVH, we conducted the microarray analysis on hESCs treated with control shRNA against Luciferase and shRNA against HERVH.
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| Overall design |
Total RNA was extracted from cells treated with control shRNA or HERVH shRNA for 5 days. 3 replicates each.
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| Contributor(s) |
Lu X, Sachs F, Ramsay L, Jacques P, Göke J, Bourque G, Ng H |
| Citation(s) |
24681886 |
| Submission date |
Nov 20, 2012 |
| Last update date |
Aug 13, 2018 |
| Contact name |
Xinyi Lu |
| Organization name |
Genome Institute of Singpaore
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| Street address |
60 Biopolis
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| City |
Singapore |
| ZIP/Postal code |
138672 |
| Country |
Singapore |
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| Platforms (1) |
| GPL10558 |
Illumina HumanHT-12 V4.0 expression beadchip |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA181953 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE42430_RAW.tar |
26.2 Mb |
(http)(custom) |
TAR |
| GSE42430_non-normalized.txt.gz |
2.0 Mb |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
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