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| Status |
Public on Nov 22, 2012 |
| Title |
Gene expression profile of KM-H2 cells conditional expressing PU.1 |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
PU.1 is an Ets family transcription factor that is essential for the differentiation of both myeloid and lymphoid cells. PU.1 is down-regulated in classical Hodgkin lymphoma cells via methylation of the PU.1 promoter. To evaluate whether down-regulation of PU.1 is essential for the growth of cHL cells, we generated KM-H2 derived cell lines conditionally express PU.1 by tet-off system (designated KM-H2tetPU.1). Conditonally expressed PU.1 by tetracycline removal induced complete growth arrest and apoptosis in KM-H2 cells. To elucidate the mechanisms underlying cell cycle arrest and apoptosis induced by PU.1, we compared gene expression profiles of KM-H2tetPU.1 cells 0, 1 and 3 days after PU.1 induction, by DNA microarray.
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| Overall design |
We extracted total RNA from KM-H2tetPU.1 cells 0, 1 and 3 days after PU.1 induction by tetracycline removal. We compared gene expression profiles of KM-H2tetPU.1 cells 0, 1 and 3 days after PU.1 induction using DNA microarray analysis. 4 independent experiments were performed with each RNA samples.
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| Contributor(s) |
Yuki H, Ueno S, Tatetsu H, Niiro H, Iino T, Endo S, Kawano Y, Komohara Y, Takeya M, Hata H, Okada S, Watanabe T, Akashi K, Mitsuya H, Okuno Y |
| Citation(s) |
23212521 |
| Submission date |
Nov 21, 2012 |
| Last update date |
Aug 13, 2018 |
| Contact name |
Yutaka Okuno |
| E-mail(s) |
yokuno@gpo.kumamoto-u.ac.jp
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| Phone |
+81-96-373-5156
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| Organization name |
Kumamoto university of Medicine
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| Department |
Department of Hematology
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| Street address |
1-1-1 Honko, Chuo Ku
|
| City |
Kumamoto |
| State/province |
Kumamoto |
| ZIP/Postal code |
860-8556 |
| Country |
Japan |
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| Platforms (1) |
| GPL10558 |
Illumina HumanHT-12 V4.0 expression beadchip |
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| Samples (12)
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| GSM1040133 |
tet-off day 0 [KM-H2tetPU.1 cells]_rep1 |
| GSM1040134 |
tet-off day 0 [KM-H2tetPU.1 cells]_rep2 |
| GSM1040135 |
tet-off day 0 [KM-H2tetPU.1 cells]_rep3 |
| GSM1040137 |
tet-off day 0 [KM-H2tetPU.1 cells]_rep4 |
| GSM1040138 |
tet-off day 1 [KM-H2tetPU.1 cells]_rep1 |
| GSM1040139 |
tet-off day 1 [KM-H2tetPU.1 cells]_rep2 |
| GSM1040140 |
tet-off day 1 [KM-H2tetPU.1 cells]_rep3 |
| GSM1040141 |
tet-off day 1 [KM-H2tetPU.1 cells]_rep4 |
| GSM1040142 |
tet-off day 3 [KM-H2tetPU.1 cells]_rep1 |
| GSM1040143 |
tet-off day 3 [KM-H2tetPU.1 cells]_rep2 |
| GSM1040144 |
tet-off day 3 [KM-H2tetPU.1 cells]_rep3 |
| GSM1040145 |
tet-off day 3 [KM-H2tetPU.1 cells]_rep4 |
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| This SubSeries is part of SuperSeries: |
| GSE42441 |
PU.1 is a potent tumor suppressor in classical Hodgkin lymphoma cells |
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| Relations |
| BioProject |
PRJNA181982 |
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