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| Status |
Public on Jan 23, 2014 |
| Title |
The Central Role of EED in the Orchestration of Polycomb Group Complexes |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Polycomb Repressive Complexes 1 and 2 (PRC1 and 2) play a critical role in the epigenetic regulation of transcription during cellular differentiation, stem cell pluripotency, and neoplastic progression1-3. Here we show that the Polycomb group protein EED, a core component of PRC2, physically interacts with and functions as part of the PRC1 complex. Components of PRC1 and PRC2 compete for EED binding. EED functions to recruit PRC1 to H3K27me3 loci and enhances PRC1 mediated H2A ubiquitin E3 ligase activity. Taken together, we uncover the integral role of EED as an epigenetic exchange factor coordinating the activities of PRC1 and 2.
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| Overall design |
EED, uH2A, RING1A, RING1B, BMI1 and H3K27Me3 ChIP-seq in EED stable knockdown and control Scramble DU145 prostate cancer cell line
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| Contributor(s) |
Cao Q, Zhao M, Yang R |
| Citation missing |
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| Submission date |
Nov 27, 2012 |
| Last update date |
May 15, 2019 |
| Contact name |
Meng Zhao |
| E-mail(s) |
meng.zhao@emory.edu
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| Phone |
404-712-8611
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| Organization name |
Emory University
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| Department |
BIOS
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| Lab |
Steve Qin
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| Street address |
1518 Clifton Rd, #369
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| City |
Atlanta |
| State/province |
GA |
| ZIP/Postal code |
30322 |
| Country |
USA |
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| Platforms (2) |
| GPL9115 |
Illumina Genome Analyzer II (Homo sapiens) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
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| Samples (14)
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| Relations |
| BioProject |
PRJNA182305 |
| SRA |
SRP017337 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE42566_RAW.tar |
3.7 Mb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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