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| Status |
Public on Aug 18, 2014 |
| Title |
FKBP5 expression in human adipose tissue increases following dexamethasone exposure and is associated with insulin resistance |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Glucocorticoid excess is linked to central obesity, adipose tissue insulin resistance and type 2 diabetes mellitus. The aim of our study was to investigate the effects of dexamethasone on gene expression in human subcutaneous and omental adipose tissue, in order to identify potential novel mechanisms and biomarkers for glucocorticoid-induced insulin resistance in adipose tissue. Dexamethasone changed the expression of 527 genes in both subcutaneous and omental adipose tissue. FKBP5 and CNR1 were the most responsive genes in both depots (~7-fold increase). Dexamethasone increased FKBP5 gene and protein expression in a dose-dependent manner in both depots, but FKBP5 protein levels were 10-fold higher in omental than subcutaneous adipose tissue. FKBP5 gene expression in subcutaneous adipose tissue was positively correlated with serum insulin, HOMA-IR and subcutaneous adipocyte diameter, while fold change in gene expression by dexamethasone was negatively correlated with clinical markers of insulin resistance, i.e. HbA1c, BMI, HOMA-IR and serum insulin. Only one gene, SERTM1, clearly differed in response to dexamethasone between the two depots. Dexamethasone at high concentrations, influences gene expression in both subcutaneous and omental adipose tissue in a similar pattern and promotes gene expression of FKBP5, a gene that may be implicated in glucocorticoid-induced insulin resistance.
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| Overall design |
Paired human subcutaneous (sc) and omental (om) adipose tissue samples obtained from 4 non-diabetic adipose tissue donors (4 M; BMI: 20.8-27.5 Kg/m2) were incubated without (Ctr) or with dexamethasone (Dex, 3 μM) for 24 h.
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| Contributor(s) |
Pereira MJ, Palming J, Svensson MK, Rizell M, Dalenbäck J, Hammar M, Svensson P, Eriksson JW |
| Citation(s) |
24997500 |
| Submission date |
Dec 13, 2012 |
| Last update date |
Feb 18, 2019 |
| Contact name |
Maria J Pereira |
| E-mail(s) |
maria.pereira@medsci.uu.se
|
| Phone |
+46 018 611 2925
|
| Organization name |
Uppsala University
|
| Department |
Department of Medical Sciences
|
| Street address |
Akademiska sjukhuset, Ing 70 3trp Lab 21
|
| City |
Uppsala |
| ZIP/Postal code |
751 85 |
| Country |
Sweden |
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| Platforms (1) |
| GPL5175 |
[HuEx-1_0-st] Affymetrix Human Exon 1.0 ST Array [transcript (gene) version] |
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| Samples (16)
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| GSM1053076 |
F1_Subcutaneous adipose tissue_dexamethasone |
| GSM1053077 |
NT02_Omental adipose tissue_control |
| GSM1053078 |
NT02_Omental adipose tissue_dexamethasone |
| GSM1053079 |
NT02_Subcutaneous adipose tissue_control |
| GSM1053080 |
NT02_Subcutaneous adipose tissue_dexamethasone |
| GSM1053081 |
NT13_Omental adipose tissue_control |
| GSM1053082 |
NT13_Omental adipose tissue_dexamethasone |
| GSM1053083 |
NT13_Subcutaneous adipose tissue_control |
| GSM1053084 |
NT13_Subcutaneous adipose tissue_dexamethasone |
| GSM1053085 |
NT17_Omental adipose tissue_control |
| GSM1053086 |
NT17_Omental adipose tissue_dexamethasone |
| GSM1053087 |
NT17_Subcutaneous adipose tissue_control |
| GSM1053088 |
NT17_Subcutaneous adipose tissue_dexamethasone |
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| Relations |
| BioProject |
PRJNA183796 |
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