|
| Status |
Public on Dec 09, 2013 |
| Title |
Small and large RNA sequencing of a Kras G12D mouse model of lung adenocarcinoma |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
Non-coding RNA profiling by high throughput sequencing
|
| Summary |
Mice bearing a G12D activating mutation in Kras consistently develop lung adenocarcinomas in a manner analogous to humans. By performing small and large RNA sequencing on KrasG12D tumors from F1 hybrid mice we were able to identify genes and microRNAs differentially expressed in these tumor samples. Quantification of reads that cover single nucleotide polymorphisms that distinguish between the parental mouse strains enabled an analysis of allele specific expression and imprinting status in these tumors.
|
| |
|
| Overall design |
mRNA and small RNA fractions of mouse lungs and lung adenocarcinomas were deep sequenced in triplicate
|
| |
|
| Contributor(s) |
Valdmanis PN, Kay MA |
| Citation(s) |
24317514 |
| Submission date |
Dec 19, 2012 |
| Last update date |
May 15, 2019 |
| Contact name |
Paul Valdmanis |
| E-mail(s) |
paulnv@uw.edu
|
| Phone |
206-221-8059
|
| Organization name |
University of Washington
|
| Department |
Medicine
|
| Lab |
Paul Valdmanis
|
| Street address |
1705 NE Pacific St, HSB J-309
|
| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98115-7720 |
| Country |
USA |
| |
|
| Platforms (2) |
| GPL9250 |
Illumina Genome Analyzer II (Mus musculus) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
|
| Samples (12)
|
|
| Relations |
| BioProject |
PRJNA184072 |
| SRA |
SRP017615 |
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