|
| Status |
Public on Apr 03, 2013 |
| Title |
Expression data from a human MLL-AF6 positive AML cell line |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
The MLL gene on chromosome 11 fuses to the AF6 gene on chromosome 6 in a balanced chromosomal translocation that is characetristic of certain adult and pediatric human leukemias. We established a murine leukemia model of MLL-AF6 using the retroviral MLL-AF6 contruct in a bone marrow transplantation system.
The ML2 human MLL-AF6 positive leukemia cell line was used for gene expression profiling to assess the transctiptional profile in MLL-AF6 leukemias.
|
| |
|
| Overall design |
RNA was extracted from the ML2 cell line cultured under standard conditionswith TRIZOL (Invitrogen), purified using RNAeasy(Qiagen) and hybridized onto Affymetrix arrays.
|
| |
|
| Contributor(s) |
Sinha A, Deshpande A, Armstrong S |
| Citation(s) |
23361907 |
| Submission date |
Dec 20, 2012 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Amit Sinha |
| E-mail(s) |
amit.sinha@childrens.harvard.edu
|
| Phone |
617-582-7579
|
| Organization name |
Dana-Farber Cancer Institute
|
| Department |
Pediatric Oncology
|
| Lab |
Armstrong Lab
|
| Street address |
44 Binney St
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02135 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
|
| Samples (1) |
|
| This SubSeries is part of SuperSeries: |
|
| Relations |
| BioProject |
PRJNA184355 |
