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| Status |
Public on Dec 25, 2012 |
| Title |
Global gene expression analysis of peripheral blood cells, pluripotent cell lines, and iPS-derived T cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Exhaustion of antigen-specific T cells represents a major challenge to adoptive immunotherapy against many types of cancer and viral infection. In an effort to overcome this problem, we reprogrammed clonally expanded antigen-specific CD8+ T cells from an HIV-1-infected patient to pluripotency. The T cell-derived induced pluripotent stem cells (T-iPSCs) were then redifferentiated into CD8+ T cells that had high proliferative capacity and elongated telomeres. To confirm that T-iPSCs were compatible to other embryonic stem cells (ESCs) but different from T cells, and that redifferentiated T cells were compatible to T cells but different from natural killer (NK) cells, we analyzed and compared the gene expression profiles of the samples by cDNA microarray. These analyses revealed that our T-iPSCs and redifferentiated T cells were essentially the same as their counterpart pluripotent stem cells and T cells, respectively.
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| Overall design |
We generated human T-iPSC lines from peripheral blood T cells of healthy donors. To confirm that established T-iPSCs were typical iPSCs, the gene expression profile of one T-iPSC clone derived from a healthy person (TkT3V1-7) was compared to those of peripheral blood T cells (CD4+ T cell and CD8+ T cell) and ESCs (KhES3). We also established T-iPSCs (H254SeVT-3) from a T-cell clone (H25-#4) which was derived from an HIV-1-infected patient. H254SeVT-3 was redifferentitated into functional T cells (reT-1 and reT-2.1) in vitro. The gene expression profiles of reT-1 and reT-2.1 were compared to those of H25-#4 and peripheral blood NK cells to clarify that the redifferentiated T cells carried the same characteristics as the original T-cell clone.
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| Contributor(s) |
Nishimura T, Kaneko S, Nakauchi H |
| Citation(s) |
23290140 |
| Submission date |
Dec 24, 2012 |
| Last update date |
Jan 23, 2019 |
| Contact name |
Toshinobu Nishimura |
| E-mail(s) |
n-toshi@ims.u-tokyo.ac.jp
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| Phone |
+81-3-5449-5331
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| Fax |
+81-3-5449-5451
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| Organization name |
The Institute of Medical Science, The University of Tokyo
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| Department |
Division of Stem Cell Therapy, Center for Stem Cell Biology and Regenerative Medicine
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| Street address |
4-6-1 Shirokanedai, Minato-ku
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| City |
Tokyo |
| ZIP/Postal code |
108-8639 |
| Country |
Japan |
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| Platforms (2) |
| GPL6480 |
Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Probe Name version) |
| GPL14550 |
Agilent-028004 SurePrint G3 Human GE 8x60K Microarray (Probe Name Version) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA184700 |
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