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| Status |
Public on Jan 05, 2013 |
| Title |
ICL0011 - Host response in human Calu-3 cells to infection with A/Vietnam/1203-CIP048_RG3/2004 (H5N1) (PB1-F2 deletion), A/Vietnam/1203-CIP048_RG3/2004 (H5N1) (PB2-627E mutant) or WT: A/Vietnam/1203/2004 (H5N1) |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Differential expression was determined in Calu-3 cells between mock infected and infection with one of 3 Influenza viruses (wild-type VN1203, VN1203 mutant PB1-F2del, VN1203 mutant PB2-627E) at different times post infection.
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| Overall design |
Purpose: To obtain samples for transcriptional analysis in triplicate using the VN1203 pathogenicity mutants PB1-F2del and PB2-627E.
Overview of Experiment:
. Time Points: 0, 3, 7, 12, 18 and 24 hrs post infection.
. There are two time points for wild type VN1203.
. Done in triplicate.
. Triplicates are defined as 3 different wells, plated at the same time using the same cell stock for all replicates.
. Time-matched mocks were done in triplicate from the same cell stock as the rest of the samples.
. Culture medium (the same as what the virus stock is in) was used for the mock infections.
Calu-3 cells were infected with A/Vietnam/1203-CIP048_RG3/2004 (H5N1) (PB1-F2 deletion), A/Vietnam/1203-CIP048_RG3/2004 (H5N1) (PB2-627E mutant) or mock infected and samples were collected at 0, 3, 7, 12, 18 and 24 hpi.
Calu-3 cells were infected with WT: A/Vietnam/1203/2004 (H5N1) and samples were collected at 7 and 24 hpi.
There are 3 mock and 3 infected replicates for each time point. Expression profiles were determined.
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| Contributor(s) |
Katze MG, Eisfeld AJ, Kawaoka Y, Fan S, Law GL, Chang J |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Dec 29, 2012 |
| Last update date |
Jan 23, 2019 |
| Contact name |
Michael Katze |
| E-mail(s) |
data@viromics.washington.edu
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| Organization name |
University of Washington
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| Department |
Microbiology
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| Lab |
Michael G. Katze, Ph.D
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| Street address |
Rosen Building 960 Republican St.
|
| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98109-4325 |
| Country |
USA |
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| Platforms (1) |
| GPL6480 |
Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Probe Name version) |
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| Samples (60)
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| Relations |
| BioProject |
PRJNA185323 |
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