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| Status |
Public on Mar 03, 2013 |
| Title |
Lineage-specific functions of Bcl6 in immunity and inflammation are mediated through distinct biochemical mechanisms |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
The transcription factor Bcl6 orchestrates the germinal center reaction through its actions in B and T cells, and regulates inflammatory signaling in macrophages. We report that genetic replacement by mutant Bcl6, which cannot bind corepressors to its BTB domain, disrupted germinal center formation and immunoglobulin affinity maturation, due to a defect in B cell proliferation and survival. In contrast, BTB loss of function had no effect on T follicular helper cell differentiation and function, nor other T helper subsets. Bcl6 null mice displayed a lethal inflammatory phenotype, whereas BTB mutant mice experienced normal healthy lives with no inflammation. Bcl6 repression of inflammatory responses in macrophages was accordingly independent of the BTB domain repressor function. Bcl6 thus mediates its actions through lineage-specific biochemical functions.
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| Overall design |
ChIP-seq for Bcl6, SMRT and BCOR in germinal center B cells
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| Contributor(s) |
Hatzi K, Melnick A |
| Citation(s) |
23455674 |
| Submission date |
Jan 08, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
Katerina Hatzi |
| E-mail(s) |
kac2029@med.cornell.edu
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| Organization name |
WCMC
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| Department |
Hematology/Oncology
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| Lab |
Ari Melnick
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| Street address |
413 E 69th Street, BB-1462
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| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10021 |
| Country |
USA |
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| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA185984 |
| SRA |
SRP018041 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE43350_RAW.tar |
1.9 Gb |
(http)(custom) |
TAR (of WIG) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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