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Series GSE43748 Query DataSets for GSE43748
Status Public on Jun 27, 2013
Title Transcriptional profiles of psychostimulant reinforcement in rats
Organism Rattus norvegicus
Experiment type Expression profiling by array
Summary Drug-induced alterations in transcriptional regulation play a central role in establishing the persistent neuroplasticities that occur during drug addiction. Additionally, changes in gene expression associated with drug administration provide valuable insight into the molecular basis of drug abuse. The molecular mechanisms that underlie susceptibility to psychostimulant addiction remain unknown. Identifying the common gene transcriptional responses to psychostimulants can provide a mechanistic insight to elucidate the molecular nature of drug dependence.
 
Overall design Male Wistar rats (4 weeks old) were acclimatized for a week to their housing conditions prior to experiments. Thereafter, they were divided into two groups: (cohort 1) 4 groups of rats (n=12 rats per group) given saline only (i.p., "drug-naive" rats); and (cohort 2): 3 groups of rats given either methamphetamine, amphetamine or methylphenidate (5 mg/kg,i.p., “drug-pretreated") for 7 days (2x daily) prior to behavioral assays. Conditioned place preference (CPP) tests were conducted a day after the final drug administration. Rats which showed CPP were evaluated for self-administration of the psychostimulants.
[Cohort 1] A day after the final self-administration test, brains of 2-3 rats from each subgroups (i.e. those which showed the most robust self-administration), as well as 5 rats from the control group were removed for microarray analysis. The striatum (rostral part of the caudate putamen and the nucleus accumbens [NAc]) and the prefrontal cortex were dissected and immediately frozen at -70°C. Total RNA was isolated and gene expression profiling was conducted on independent pools of total RNA from 2-5 animals per group.
[Cohort 2] A day after the final self-administration test, brains of 3 rats from each subgroups (i.e. those which showed the most robust self-administration), as well as 3 rats from the control group were removed for microarray analysis. The striatum (rostral part of the caudate putamen and the nucleus accumbens [NAc]) and the prefrontal cortex were dissected and immediately frozen at -70°C. Total RNA was isolated and gene expression profiling was conducted on independent pools of total RNA from three animals per group.
 
Contributor(s) Noh M, Cheong J
Citation(s) 23783774
Submission date Jan 25, 2013
Last update date Jul 31, 2017
Contact name Minsoo Noh
E-mail(s) minsoo@alum.mit.edu
Phone 82-2-880-2481
Organization name Seoul National University
Department Pharmacy
Lab Systems Pharmacology
Street address 1 Gwanak-ro, Gwanak-gu
City Seoul
ZIP/Postal code 151-742
Country South Korea
 
Platforms (1)
GPL1355 [Rat230_2] Affymetrix Rat Genome 230 2.0 Array
Samples (63)
GSM1070248 Cohort1-Prefrontal Cortex_Control group_Sample 1
GSM1070249 Cohort1-Prefrontal Cortex_Control group_Sample 2
GSM1070250 Cohort1-Prefrontal Cortex_Control group_Sample 3
Relations
BioProject PRJNA187273

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE43748_RAW.tar 241.8 Mb (http)(custom) TAR (of CEL, CHP)
Processed data included within Sample table
Processed data provided as supplementary file
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