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| Status |
Public on Jan 21, 2015 |
| Title |
Global gene expression analysis of human iPSC-derived Retinal Pigmented Epithelial (RPE) cells from LCA patients and unaffected persons |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Our purpose was to investigate genes and molecular mechanisms involved in patients with Leber congenital amaurosis (LCA). Fibroblasts from two unrelated clinically-identified patients (Coriell) were reprogrammed to pluripotency by retroviral transduction. These human induced Pluripotent Stem Cells (hiPSCs) were differentiated into neural stem cells (NSC) that mimicked the neural tube stage and retinal pigmented epithelial (RPE) cells that could be targeted by the disease. A genome wide transcriptome analysis was performed with Affymetrix Exon Array GeneChip®, comparing LCA-hiPSCs derivatives to controls. The aim was to identify differentially expressed genes which may be associated with early developmental defect before the establishment of mature retinal circuitry.
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| Overall design |
We analyzed iPSC-derived retinal pigmented epithelial (RPE) cells from LCA patient's fibroblast (n=2) and iPSC-derivedretinal pigmented epithelial (RPE) cells from healthy people fibroblast (n=2). A total of 13 samples were analyzed : 9 RPE cells derived from iPSC LCA and 4 RPE cells derived from wild-type iPSC.
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| Contributor(s) |
Lustremant C, de la Grange P, Monville C |
| Citation(s) |
23663011 |
| Submission date |
Jan 30, 2013 |
| Last update date |
Feb 18, 2019 |
| Contact name |
Pierre de la Grange |
| E-mail(s) |
pierre.delagrange@genosplice.com
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| Organization name |
GenoSplice technology
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| Department |
Paris Biotech Santé
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| Street address |
29, rue du Faubourg Saint-Jacques
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| City |
Paris |
| ZIP/Postal code |
75014 |
| Country |
France |
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| Platforms (1) |
| GPL5175 |
[HuEx-1_0-st] Affymetrix Human Exon 1.0 ST Array [transcript (gene) version] |
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| Samples (13)
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| GSM1074490 |
hiPSC GM03852cl1-derived Retinal Pigmented Epithelial (RPE) cells at passage 1, biological rep1 |
| GSM1074491 |
hiPSC GM03852cl1-derived Retinal Pigmented Epithelial (RPE) cells at passage 2, biological rep2 |
| GSM1074492 |
hiPSC GM03852cl1-derived Retinal Pigmented Epithelial (RPE) cells at passage 3, biological rep3 |
| GSM1074493 |
hiPSC GM05300cl4-derived Retinal Pigmented Epithelial (RPE) cells at passage 3, biological rep1 |
| GSM1074494 |
hiPSC GM05300cl4-derived Retinal Pigmented Epithelial (RPE) cells at passage 3, biological rep2 |
| GSM1074495 |
hiPSC GM05300cl4-derived Retinal Pigmented Epithelial (RPE) cells at passage 3, biological rep3 |
| GSM1074496 |
hiPSC GM05300cl5-derived Retinal Pigmented Epithelial (RPE) cells at passage 3, biological rep1 |
| GSM1074497 |
hiPSC GM05300cl5-derived Retinal Pigmented Epithelial (RPE) cells at passage 1, biological rep2 |
| GSM1074498 |
hiPSC GM05300cl5-derived Retinal Pigmented Epithelial (RPE) cells at passage 3, biological rep3 |
| GSM1074499 |
hiPSC GM4603cl27-derived Retinal Pigmented Epithelial (RPE) cells at passage 1, biological rep1 |
| GSM1074500 |
hiPSC GM4603cl27-derived Retinal Pigmented Epithelial (RPE) cells at passage 2, biological rep2 |
| GSM1074501 |
hiPSC GM4603cl28-derived Retinal Pigmented Epithelial (RPE) cells at passage 2, biological rep1 |
| GSM1074502 |
hiPSC GM4603cl28-derived Retinal Pigmented Epithelial (RPE) cells at passage 3, biological rep2 |
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| This SubSeries is part of SuperSeries: |
| GSE43946 |
Human induced pluripotent stem cells reveal early developmental molecular correlates with a probable Leber congenital amaurosis type I |
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| Relations |
| BioProject |
PRJNA188201 |
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