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| Status |
Public on Feb 07, 2013 |
| Title |
In vivo NCL-targeting affects breast cancer aggressiveness through miRNA regulation [RNA-seq] |
| Organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
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| Summary |
Numerous studies have described the altered expression and the causal role of miRNAs in human cancer. However, to date efforts to modulate miRNA levels for therapeutic purposes have been challenging to implement. Here, we find that Nucleolin (NCL), a major nucleolar protein, post-transcriptionally regulates the expression of a specific subset of miRNAs, including miR-21, miR-221, miR-222, and miR-103, causally involved in breast cancer initiation, progression and drug-resistance. We also show that NCL is commonly overexpressed in human breast tumors, and its expression correlates with that of NCL-dependent miRNAs. Finally, this study indicates that NCL-binding guanosine-rich aptamers affect the levels of NCL-dependent miRNAs and their target genes, reducing breast cancer cell aggressiveness, both in vitro and in vivo. These findings illuminate a path to novel therapeutic approaches based on NCL-targeting aptamers for the modulation of miRNA expression in the treatment of breast cancer.
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| Overall design |
Identification of NCL regulated miRNAs by using miRNA high-throughput sequencing of HeLa cells stably expressing double-strand (ds) interfering RNA against NCL or scrambled sequences (sh-NCL or sh-Scr).
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| Contributor(s) |
Pichiorri F, Palmieri D, De Luca L, Consiglio J, You J, Rocci A, Talabere T, Piovan C, Lagana A, Cascione L, Guan J, Gasparini P, Balatti V, Coppola V, Hofmeister CC, Marcucci G, Byrd J, Volinia S, Shapiro CL, Freitas MA, Croce CM |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Feb 04, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
Luciano Cascione |
| E-mail(s) |
luciano.cascione@ior.usi.ch
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| Organization name |
IOR
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| Department |
Lymphoma & Genomics Research Program
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| Street address |
Via Chiesa 5
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| City |
Bellinzona |
| State/province |
Ticino |
| ZIP/Postal code |
6500 |
| Country |
Switzerland |
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| Platforms (1) |
| GPL13393 |
AB SOLiD 4 System (Homo sapiens) |
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| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE41973 |
In vivo NCL-targeting affects breast cancer aggressiveness through miRNA regulation |
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| Relations |
| BioProject |
PRJNA188684 |
| SRA |
SRP018427 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE44052_HeLa_sh-NCL_miR_PASS_counts.ARPKM.txt.gz |
8.9 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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