|
| Status |
Public on Dec 05, 2013 |
| Title |
Cellular generation of 5-Hydroxymethylcytosine by redox-active chemicals via an unprecedent non-enzymatic mechanism |
| Organism |
Homo sapiens |
| Experiment type |
Methylation profiling by high throughput sequencing
Expression profiling by high throughput sequencing
|
| Summary |
We proposed that besides TET family dioxygenase oxidizing 5mC to 5hmC, there is a non-enzyme pathway which is due to hydroxyl radica l(OH) or OH-like species also involvement in demethylation of 5mC forming 5hmC. This pathway includes classical fenton reagents such as H2O2 and Fe2+, and more important redox-activity quinoid compounds, especially, tetrachloro-1,4-benzoquinone (TCBQ), which was reported producing hydroxyl radicals independent of transition metal
|
| |
|
| Overall design |
Examination of 5hmC and transcriptome levels with TCBQ and DMSO in human MRC-5 cell lines
|
| |
|
| Contributor(s) |
Yang Y, Wang H |
| Citation(s) |
24214992 |
| Submission date |
Feb 21, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
Zechen Chong |
| Organization name |
Beijing Institute of Genomics
|
| Street address |
NO. 7 Beitucheng West Road
|
| City |
Beijing |
| ZIP/Postal code |
100029 |
| Country |
China |
| |
|
| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
|
| Samples (4)
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|
| Relations |
| BioProject |
PRJNA190013 |
| SRA |
SRP018778 |
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