|
| Status |
Public on Sep 20, 2013 |
| Title |
Genome-wide analysis of dFOXO binding sites in Drosophila |
| Organism |
Drosophila melanogaster |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
To understand how reduced insulin/IGF-1 signaling extends Drosophila lifespan through its downstream transcription factor dFOXO. We conducted ChIP analysis with a dFOXO antibody followed by Illumina high-throughput sequencing from chico heterozygous mutants, which are long-lived and normal sized, and from adult flies with ablated insulin producing cells (IPCs), which are also long-lived. dFOXO bound at promoters of 273 genes common among these genotypes, thus potentially enriching for shared factors in control of aging.
|
| |
|
| Overall design |
Two replicates were sequenced from chico heterozygous mutants and IPC ablated flies.
|
| |
|
| Contributor(s) |
Bai H, Tatar M |
| Citation(s) |
24244197 |
| Submission date |
Feb 26, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
Hua Bai |
| E-mail(s) |
hua_bai@brown.edu
|
| Organization name |
Brown University
|
| Department |
EEB
|
| Lab |
Marc Tatar Lab
|
| Street address |
34 Olive St, BMC502
|
| City |
Providence |
| State/province |
RI |
| ZIP/Postal code |
02912 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL11203 |
Illumina Genome Analyzer IIx (Drosophila melanogaster) |
|
| Samples (8)
|
|
| Relations |
| BioProject |
PRJNA191574 |
| SRA |
SRP018869 |
Less...
More...