|
| Status |
Public on Feb 27, 2013 |
| Title |
Comparative study of deoxynivalenol and 15-acetyldeoxynivalenol on gene expression in the human cell line Caco-2 |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
To screen cellular effects on Caco-2 cells by deoxynivalenol (DON) and 15-acetyldeoxynivalenol (15ADON), toxin-treated Caco-2 cells were analyzed by DNA microarray. Exposure to either toxin induced up- or downregulation of genes in Caco-2 cells. Upregulation of 1290 and 3238 genes was observed for the DON- and 15ADON-treated groups, respectively, after a 60-min incubation period. This represented a greater than 1.5-fold change relative to the 0-min exposure (control) group. Five hundred and sixty-one genes showed a 1.5-fold upregulation in the both the DON- and 15ADON-treated groups. Similarly, 13 genes and 105 genes were upregulated with a log2 ratio > 2 (4-fold change) in the DON- and 15ADON-treated groups, respectively.
The most remarkable gene upregulation corresponded to the inflammatory chemokine, IL-8, which showed a greater than 4-fold upregulation in response to both treatments.
|
| |
|
| Overall design |
Deoxynivalenol and 15-acetyldeoxynivalenol at concentrations of 1 ug/mL were added separately to the AP chamber, and cells were harvested with 0.5% trypsin-EDTA after incubation for 0, 5, 30, and 60 min. Toxin-treated Caco-2 cells were analyzed by DNA microarray to screen for changes in gene expression in response to deoxynivalenol or 15-acetyldeoxynivalenol.
|
| |
|
| Contributor(s) |
Kadota T, Sugita-Konishi Y |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Feb 27, 2013 |
| Last update date |
Oct 11, 2016 |
| Contact name |
Tomoyuki Kadota |
| E-mail(s) |
Tomoyuki_Kadota@kirin.co.jp
|
| Organization name |
Kirin Company, Limited
|
| Department |
Research & Development Division
|
| Lab |
Central Laboratories for Key Technologies
|
| Street address |
1-13-5, Fukuura Kanazawa-ku
|
| City |
Yokohama |
| State/province |
Kanagawa |
| ZIP/Postal code |
236-0004 |
| Country |
Japan |
| |
|
| Platforms (1) |
| GPL14550 |
Agilent-028004 SurePrint G3 Human GE 8x60K Microarray (Probe Name Version) |
|
| Samples (8)
|
|
| Relations |
| BioProject |
PRJNA191081 |
Less...
More...