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Series GSE45024 Query DataSets for GSE45024
Status Public on Apr 18, 2013
Title Genome-wide maps of chromatin state for HIRA, UBN1 and ASF1a
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary The HIRA chaperone complex, comprised of HIRA, UBN1 and CABIN1, collaborates with histone-binding protein ASF1a to incorporate histone variant H3.3 into chromatin in a DNA replication-independent manner. To better understand its function and mechanism, we integrated HIRA, UBN1, ASF1a and histone H3.3 ChIP-seq and gene expression analyses. Most HIRA-binding sites co-localize with UBN1, ASF1a and H3.3 at active promoters and active and weak/poised enhancers. At promoters, binding of HIRA/UBN1/ASF1a correlates with the level of gene expression. HIRA is required for deposition of histone H3.3 at its binding sites. There are marked differences in nucleosome and co-regulator composition at different classes of HIRA-bound regulatory site. Underscoring this, we report novel physical interactions between the HIRA complex and transcription factors, a chromatin insulator and an ATP-dependent chromatin-remodelling complex. Our results map the distribution of the HIRA chaperone across the chromatin landscape and point to different interacting partners at functionally distinct regulatory sites.
 
Overall design Examination of 3 histone chaperone proteins in HeLa cells
 
Contributor(s) Pchelintsev NA, McBryan T, Adams PD
Citation(s) 23602572
Submission date Mar 11, 2013
Last update date May 15, 2019
Contact name Peter D. Adams
Organization name University of Glasgow, Beatson Institute for Cancer Research
Street address Switchback Rd, Bearsden
City Glasgow
ZIP/Postal code G61 1BD
Country United Kingdom
 
Platforms (1)
GPL10999 Illumina Genome Analyzer IIx (Homo sapiens)
Samples (4)
GSM1095933 ASF1a ChIP
GSM1095934 HIRA ChIP
GSM1095935 input DNA
This SubSeries is part of SuperSeries:
GSE45025 Placing the HIRA histone chaperone complex in the chromatin landscape
Relations
BioProject PRJNA192852
SRA SRP019255

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE45024_RAW.tar 1.3 Gb (http)(custom) TAR (of BED, BIGWIG, TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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