|
| Status |
Public on Mar 19, 2013 |
| Title |
Genome-wide analysis of gene expression in murine breast fibroblasts from normal tissue, hyperplasia, adenoma and carcinoma (MMTV-PyMT model) |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Analysis of differences at gene expression level of hpvE6 immortalized fibroblasts isolated from normal mammary glands and from hyperplasia, adenoma and carcinoma stages using the MMTV-PyMT model (FVB/N background). Analysis demonstrated the activation of specific transcriptional programs in fibroblasts from later stages.
|
| |
|
| Overall design |
Total RNA obtained from isolated hpvE6 immortalized fibroblasts from normal mammary gland (NF) and from different stages of tumour development using the MMTV-PyMT murine breast cancer model. Stages were hyperplasia (HpAF), adenoma (AdAF) and carcinoma (CAF). Fibroblasts were seeded in a deformable Matrigel:collagen I matrix and total RNA isolated 72h later.
|
| |
|
| Contributor(s) |
Calvo F, Sahai E |
| Citation(s) |
23708000 |
| Submission date |
Mar 18, 2013 |
| Last update date |
Jun 14, 2018 |
| Contact name |
Fernando Calvo |
| E-mail(s) |
fernando.calvo@cancer.org.uk
|
| Organization name |
Cancer Research UK
|
| Department |
London Research Institute
|
| Lab |
Tumour Cell Biology
|
| Street address |
44 Lincoln's Inn Fields
|
| City |
London |
| ZIP/Postal code |
WC2A 3LY |
| Country |
United Kingdom |
| |
|
| Platforms (1) |
| GPL6885 |
Illumina MouseRef-8 v2.0 expression beadchip |
|
| Samples (16)
|
|
| Relations |
| BioProject |
PRJNA193324 |
Less...
More...