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Status |
Public on Aug 15, 2020 |
Title |
Sacharomyces cerevisiae Transcript Isoform mapping (rrp6) |
Organism |
Saccharomyces cerevisiae |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
Application of TIF-Seq to mutants of the molecular components involved in transcription process.
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Overall design |
Application of TIF-Seq (Transcript IsoForm Sequencing) to S.cerevisiae. The method was applied to simultaneously identify the 5’ capped mRNA transcription start site and the 3’polyadenylation site in delta rrp6 mutant strain grown in glucose media [rrp6, 2 biological replicates (bio) and 2 independent library preparations, technical replicates(lib)].
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Contributor(s) |
Pelechano V, Wei W, Steinmetz LM |
Citation missing |
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Submission date |
Apr 17, 2013 |
Last update date |
Aug 15, 2020 |
Contact name |
Wu Wei |
E-mail(s) |
wuwei@embl.de
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Organization name |
EMBL Heidelberg
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Department |
Genome Biology Unit
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Lab |
Steinmetz Lab
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Street address |
EMBL Heidelberg,Meyerhofstraße 1
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City |
Heidelberg |
ZIP/Postal code |
69117 |
Country |
Germany |
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Platforms (1) |
GPL13821 |
Illumina HiSeq 2000 (Saccharomyces cerevisiae) |
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Samples (4)
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Relations |
BioProject |
PRJNA197347 |
SRA |
SRP021165 |