|
| Status |
Public on Dec 31, 2013 |
| Title |
Widespread Transcription beyond mRNA 3’ Ends Yields Abundant Regulatory RNAs |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Through RNA-seq analyses of nascent transcripts, we found large numbers of RNA transcripts that extend beyond the 3’ ends of protein-coding genes; we refer to these extended transcripts as geRNAs. These findings demonstrate that transcription of most human protein-coding genes does not terminate within close proximity to poly(A) signals; rather, it terminates at sites far beyond these signals (up to 50 kb).
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| Overall design |
Examination of different RNA species in HelaS3 cells by strand-specific RNA-seq. The 4sU-labeled RNA was isolated by using organomercurial affinity matrix.
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| Contributor(s) |
Huang SS, Xue GG, Chen CC |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Apr 19, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
Shenglin Huang |
| Organization name |
Fudan University
|
| Street address |
270 Dongan
|
| City |
Shanghai |
| ZIP/Postal code |
200032 |
| Country |
China |
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| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA198440 |
| SRA |
SRP021214 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE46228_4sU_RNA_minus.bigwig |
446.5 Mb |
(ftp)(http) |
BIGWIG |
| GSE46228_4sU_RNA_plus.bigwig |
476.8 Mb |
(ftp)(http) |
BIGWIG |
| GSE46228_Supplementary_table.txt.gz |
238.4 Kb |
(ftp)(http) |
TXT |
| GSE46228_cyto_RNA_minus.bigwig |
66.7 Mb |
(ftp)(http) |
BIGWIG |
| GSE46228_cyto_RNA_plus.bigwig |
62.3 Mb |
(ftp)(http) |
BIGWIG |
| GSE46228_nucleus_RNA_minus.bigwig |
234.2 Mb |
(ftp)(http) |
BIGWIG |
| GSE46228_nucleus_RNA_plus.bigwig |
243.7 Mb |
(ftp)(http) |
BIGWIG |
| GSE46228_polyA_RNA_minus.bigwig |
129.9 Mb |
(ftp)(http) |
BIGWIG |
| GSE46228_polyA_RNA_plus.bigwig |
136.4 Mb |
(ftp)(http) |
BIGWIG |
SRA Run Selector |
| Processed data are available on Series record |
| Raw data are available in SRA |
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