|
| Status |
Public on Apr 24, 2013 |
| Title |
Engineering Bone Tissue Substitutes from Human Induced Pluripotent Stem Cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Global gene expression data of human embryonic stem cell-, human induced pluripotent stem cell- and bone marrow-derived mesenchymal progenitor cells before and after culture onto osteoinductive scaffolds in perfusion bioreactors. The hypothesis tested in the present study was that perfusion culture in bioreactors influenced the expression levels of several genes involved in proliferation and osteogenic differentiation. Results provide important information of the response of human embryonic stem cell-, human induced pluripotent stem cell- and bone marrow-derived mesenchymal progenitor cell to osteogenic stimulation under perfusion cultures, such as genes involved in cell proliferation and division as well as osteogenic differentiation and bone development.
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| |
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| Overall design |
Total RNA obtained from human embryonic stem cell-, human induced pluripotent stem cell- and bone marrow-derived mesenchymal progenitor cells before and after culture under osteogenic conditions in perfusion bioreactors for 5 weeks.
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| |
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| Contributor(s) |
de Peppo GM, Campos IM, Kahler D, Alsalman D, Shang L, Vunjak-Novakovic G, Marolt D |
| Citation(s) |
23653480 |
| Submission date |
Apr 23, 2013 |
| Last update date |
Aug 13, 2018 |
| Contact name |
Giuseppe Maria de Peppo |
| E-mail(s) |
gmdepeppo@nyscf.org
|
| Organization name |
The New York Stem Cell Foundation
|
| Department |
Research and Development
|
| Lab |
Bone Regeneration and Engineering
|
| Street address |
3960 Broadway
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10032 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL10558 |
Illumina HumanHT-12 V4.0 expression beadchip |
|
| Samples (12)
|
| GSM1128574 |
Human bone marrow-derived mesenchymal cells (donor 1) |
| GSM1128575 |
Human bone marrow-derived mesenchymal cells (donor 2) |
| GSM1128576 |
Human embryonic stem cell-derived mesenchymal cells (H9) |
| GSM1128577 |
Human embryonic stem cell-derived mesenchymal cells (H13) |
| GSM1128578 |
Human induced pluripotent stem cell-derived mesenchymal cells (1013A) |
| GSM1128579 |
Human induced pluripotent stem cell-derived mesenchymal cells (BC1) |
| GSM1128580 |
Human induced pluripotent stem cell-derived mesenchymal cells (11c) |
| GSM1128581 |
Human bone marrow-derived mesenchymal cells (donor 1)-5 weeks bioreactor |
| GSM1128582 |
Human embryonic stem cell-derived mesenchymal cells (H9)-5 weeks bioreactor |
| GSM1128583 |
Human embryonic stem cell-derived mesenchymal cells (H13)-5 weeks bioreactor |
| GSM1128584 |
Human induced pluripotent stem cell-derived mesenchymal cells (1013A)-5 weeks bioreactor |
| GSM1128585 |
Human induced pluripotent stem cell-derived mesenchymal cells (BC1)-5 weeks bioreactor |
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| Relations |
| BioProject |
PRJNA198724 |
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