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| Status |
Public on Apr 24, 2013 |
| Title |
Isolation and Characterization of a Novel Population of Pluripotent Stem Cells Derived from Human Adipose Tissue |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Transcriptional comparison of two subpopulations of stem cells isolated from lipoaspirated human adipose tissue, profiling Adipose Stem Cells (ASCs) and Multilineage Differentiating Stress-Enduring Adipose Tissue cells (MUSE-ATs). Experiments focused in the isolation and characterization of a new population of adipose tissue (AT) derived pluripotent stem cells, termed MUSE-ATs, which are isolated using severe cellular stress conditions, including long-term exposure to the proteolytic enzyme collagenase, serum deprivation, low temperatures and hypoxia. Under these conditions, a homogenous, highly purified population of Muse-AT cells is isolated without the utilization of cell sorting methods. The goal of this experiment was to characterize MUSE-ATs, as well as compare them to the endogenous subpopulation of ASCs for reference.
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| Overall design |
Single condition experiment, MUSE-ATs vs. ASCs. Biological replicates: 3 MUSE-ATs replicates, 3 ASCs replicates for reference.
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| Contributor(s) |
Heneidi SG, Simerman AA, Keller E, Singh P, Le X, Chazenbalk G |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Apr 24, 2013 |
| Last update date |
Oct 11, 2016 |
| Contact name |
Saleh Gamal Heneidi |
| E-mail(s) |
sheneidi@gmail.com
|
| Organization name |
Georgetown University Medical Center
|
| Department |
Department of Pharmacology and Physiology
|
| Street address |
3900 Reservoir Rd, NW, SE404 Med-Dent Building
|
| City |
Washington |
| State/province |
DC |
| ZIP/Postal code |
20007 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL14550 |
Agilent-028004 SurePrint G3 Human GE 8x60K Microarray (Probe Name Version) |
|
| Samples (6)
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| GSM1129047 |
Multilineage Differentiating Stress-Enduring-Adipose Tissue Cell Replicate 1 |
| GSM1129048 |
Multilineage Differentiating Stress-Enduring-Adipose Tissue Cell Replicate 2 |
| GSM1129049 |
Multilineage Differentiating Stress-Enduring-Adipose Tissue Cell Replicate 3 |
|
| Relations |
| BioProject |
PRJNA198802 |
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