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| Status |
Public on Aug 05, 2013 |
| Title |
High-throughput RNA sequencing of human preovulatory cumulus and mural granulosa cells (mRNA) |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The granulosa cells in the mammalian ovarian follicle respond to gonadotropin signalling and are involved in the processes of folliculogenesis and oocyte maturation. Studies on gene expression and regulation in human granulosa cells are of interest due to their potential for estimating the oocyte viability and IVF success. The current study determined the mRNA profile by deep sequencing of the two intrafollicular somatic cell types: mural and cumulus granulosa cells isolated from women undergoing controlled ovarian stimulation and in vitro fertilization.
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| Overall design |
Paired cumulus and mural granulosa samples were analysed from 3 women participating in IVF procedure. Differential gene expression study was performed. The identified gene expression profile was also used for predicting targets for miRNAs that were also identified from the same samples (GSE46489).
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| Contributor(s) |
Velthut-Meikas A, Simm J |
| Citation(s) |
23660593 |
| Submission date |
Apr 29, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
Agne Velthut-Meikas |
| Organization name |
Tallinn University of Technology
|
| Department |
Department of Chemistry and Biotechnology
|
| Street address |
Akadeemia tee 15
|
| City |
Tallinn |
| ZIP/Postal code |
12618 |
| Country |
Estonia |
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| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
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| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE46508 |
Small RNA-seq of human granulosa cells reveals miRNAs in FSHR and aromatase genes |
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| Relations |
| BioProject |
PRJNA200696 |
| SRA |
SRP021912 |
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