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| Status |
Public on Jan 01, 2014 |
| Title |
Selective suppression of endothelial cytokine production by progesterone receptor [RNA-seq] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Steroid hormones are well-recognized suppressors of the inflammatory response, however, their cell- and tissue-specific effects in the regulation of inflammation are far less understood, particularly for the sex-related steroids. To determine the contribution of progesterone in the endothelium, we have characterized and validated an in vitro culture system in which human umbilical vein endothelial cells constitutively express human progesterone receptor (PR). Using next generation RNA-sequencing, we identified a selective group of cytokines that are suppressed by progesterone both under physiological conditions and during pathological activation by lipopolysaccharide. In particular, IL-6, IL-8, CXCL2/3, and CXCL1 were found to be direct targets of PR, as determined by ChIP-sequencing. Regulation of these cytokines by progesterone was also confirmed by bead-based multiplex cytokine assays and quantitative PCR. These findings provide a novel role for PR in the direct regulation of cytokine levels secreted by the endothelium. They also suggest that progesterone-PR signaling in the endothelium directly impacts leukocyte trafficking in PR-expressing tissues
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| Overall design |
Examination of PR target genes in human umbilical vein endothelial cells (HUVECs) using RNA-seq
PR infected only (PR);
PR infected followed by ligand treatment (PR+P);
PR infected followed by 4h LPS treatment (PR+LPs_4h);
PR infected followed by 8h LPS treatment (PR+LPs_8h);
PR infected followed by 4h LPS and progesterone treatment (PR+LPS+P_4h);
PR infected followed by 8h LPS and progesterone treatment (PR+LPS+P_8h)
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| Contributor(s) |
Goddard LM, Ton AN, Org T, Mikkola HK, Iruela-Arispe ML |
| Citation missing |
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| Submission date |
Apr 30, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
Lauren Goddard |
| Organization name |
University of California-Los Angeles
|
| Department |
Molecular, Cell, and Developmental Biology
|
| Lab |
Dr. Luisa Iruela-Arispe
|
| Street address |
621 Charles E. Young Dr. S.
|
| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90095 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
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| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE46503 |
Selective suppression of endothelial cytokine production by progesterone receptor |
|
| Relations |
| BioProject |
PRJNA200693 |
| SRA |
SRP021908 |
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