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| Status |
Public on Apr 25, 2014 |
| Title |
SIRT1 but not its increased expression is essential for lifespan extension in caloric restricted mice. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
The SIRT1 deacetylase is one of the best-studied potential mediators of some of the anti-aging effects of calorie restriction (CR); but its role in CR-dependent lifespan extension has not been demonstrated. We previously found that mice lacking both copies of SIRT1 displayed a shorter median lifespan than wild type mice on an ad libitum diet. Here we report that median lifespan extension in CR heterozygote SIRT1+/- mice was identical (51%) to that observed in wild type mice but SIRT1+/- mice displayed a higher frequency of some certain pathologies. Although larger studies in different genetic backgrounds are necessary , these results provide strong initial evidence for the requirement of SIRT1 for the anti-aging effects of CR, but suggest that its high expression is not required for CR-induced lifespan extension.
Key words: SIRT1, caloric restriction, lifespan, anti-aging
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| Overall design |
2-5 month old male mice of 3 different genotypes (SIRT1+/+, SIRT1+/-, and SIRT1-/-) that had normal, reduced or no expression of SIRT1 were treated with either a 40% caloric restricted diet (CR) or an ad libitum diet (AL). 2-4 replicates of each experimental condition were used in the analysis.
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| Contributor(s) |
Mercken EM, Hu J, Krzysik-Walker S, Wei M, Li Y, McBurney MW, de Cabo R, Longo VD |
| Citation(s) |
23941528 |
| Submission date |
May 14, 2013 |
| Last update date |
Jun 22, 2020 |
| Contact name |
Supriyo De |
| Organization name |
NIA-IRP, NIH
|
| Department |
Laboratory of Genetics and Genomics
|
| Lab |
Computational Biology & Genomics Core
|
| Street address |
251 Bayview Blvd
|
| City |
Baltimore |
| State/province |
Maryland |
| ZIP/Postal code |
21224 |
| Country |
USA |
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| Platforms (1) |
| GPL6885 |
Illumina MouseRef-8 v2.0 expression beadchip |
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| Samples (18)
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| GSM1139917 |
Liver-Caloric Restriction- Wt-replcate 1 |
| GSM1139918 |
Liver-Caloric Restriction- Wt-replcate 2 |
| GSM1139919 |
Liver-Caloric Restriction- Homozygous knockout-replicate 1 |
| GSM1139920 |
Liver-Caloric Restriction- Homozygous knockout-replicate 2 |
| GSM1139921 |
Liver-Caloric Restriction- Homozygous knockout-replicate 3 |
| GSM1139922 |
Liver-Caloric Restriction- Heterozygous knockout-replicate 1 |
| GSM1139923 |
Liver-Caloric Restriction- Wt-replcate 3 |
| GSM1139924 |
Liver-Caloric Restriction- Heterozygous knockout-replicate 2 |
| GSM1139925 |
Liver-Caloric Restriction- Heterozygous knockout-replicate 3 |
| GSM1139926 |
Liver-Ad Lib- Homozygous knockout-replicate 1 |
| GSM1139927 |
Liver-Ad Lib- Heterozygous knockout-replicate 1 |
| GSM1139928 |
Liver-Ad Lib- Homozygous knockout-replicate 2 |
| GSM1139929 |
Liver-Ad Lib-Wt-replcate 1 |
| GSM1139930 |
Liver-Ad Lib- Homozygous knockout-replicate 3 |
| GSM1139931 |
Liver-Ad Lib- Heterozygous knockout-replicate 2 |
| GSM1139932 |
Liver-Ad Lib-Wt-replcate 2 |
| GSM1139933 |
Liver-Ad Lib-Wt-replcate 3 |
| GSM1139934 |
Liver-Ad Lib- Homozygous knockout-replicate 4 |
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| Relations |
| BioProject |
PRJNA202976 |
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