|
| Status |
Public on Aug 13, 2014 |
| Title |
Genome-wide analysis of the effects of the novel mithramycin analogue DIG-MSK on human A2780 ovarian carcinoma cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
The effects of demycarosyl-3D-β-D-digitoxosyl-mithramycin SK (DIG-MSK; EC-8042), a novel analogue of the antitumor antibiotic mithramycin A, on gene transcription were examined in human A2780 ovarian carcinoma cells. DIG-MSK down-regulated a different set of genes depending on the drug concentration. Moreover, several genes were significantly up-regulated. These results are rationalized in terms of DIG-MSK competition with Sp1 transcription factor for binding to consensus C/G-rich tracts encompassed in gene promoters.
|
| |
|
| Overall design |
Human A2780 ovarian carcinoma cells were treated with either 8 nM or 80 nM DIG-MSK for 24 h, and RNA was extracted from treated cells as well as from untreated (control) cells.
|
| |
|
| Contributor(s) |
Portugal J, Vizcaíno C, Morís F |
| Citation(s) |
25110883 |
| Submission date |
May 14, 2013 |
| Last update date |
Nov 27, 2018 |
| Contact name |
José Portugal |
| E-mail(s) |
jpmbmc@ibmb.csic.es
|
| Organization name |
Instituto de Biologia Molecular de Barcelona-CSIC
|
| Department |
Molecular Genomics
|
| Street address |
Parc Cientific de Barcelona, Baldiri Reixac, 10
|
| City |
Barcelona |
| ZIP/Postal code |
E-08028 |
| Country |
Spain |
| |
|
| Platforms (1) |
| GPL13607 |
Agilent-028004 SurePrint G3 Human GE 8x60K Microarray (Feature Number version) |
|
| Samples (9)
|
|
| Relations |
| BioProject |
PRJNA203041 |
Less...
More...