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| Status |
Public on Feb 27, 2014 |
| Title |
LRH-1 governs vital transcriptional programs in endocrine sensitive and resistant breast cancer cells: LRH-1 ChIP-seq |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Tumor characteristics are decisive in the determination of treatment strategy for breast cancer patients. Patients with estrogen receptor a(ERa)-positive breast cancer can benefit from long-term hormonal treatment. Nonetheless, the majority of patients will develop resistance to these therapies. Here, we investigated the role of the nuclear receptor liver receptor homolog-1 (LRH-1, NR5A2) in anti-estrogen (AE) sensitive and resistant breast cancer cells. We identified genome-wide LRH-1 binding sites using ChIP-seq, uncovering preferential binding to regions distal to transcriptional start sites (TSS). We further characterized these LRH-1 binding sites by integrating overlapping layers of specific chromatin marks, revealing that many LRH-1 binding sites are active and could be involved in long-range enhancer-promoter looping. Combined with transcriptome analysis of LRH-1 depleted cells, these results show that LRH-1 regulates specific subsets of genes involved in cell proliferation in AE-sensitive and AE-resistant breast cancer cells. Furthermore, the LRH-1 transcriptional program is highly associated with a signature of poor outcome and high-grade breast cancer tumors in vivo. Herein we report the genome-wide location and molecular function of LRH-1 in breast cancer cells and reveal its therapeutic potential for the treatment of breast cancers, notably for tumors resistant to treatments currently used in therapies.
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| |
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| Overall design |
ChIP-seq examination of LRH-1 binding sites with specific chromatin marks in MCF7 breast cancer cells.
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| |
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| Contributor(s) |
Bianco S, Gevry N |
| Citation(s) |
24520076 |
| Submission date |
May 16, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
Nicolas Gévry |
| E-mail(s) |
nicolas.gevry@usherbrooke.ca
|
| Organization name |
Université de Sherbrooke
|
| Street address |
2500 boulevard de l'université
|
| City |
Sherbrooke |
| State/province |
Québec |
| ZIP/Postal code |
J1K 2R1 |
| Country |
Canada |
| |
|
| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
|
| Samples (4)
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| This SubSeries is part of SuperSeries: |
| GSE54892 |
LRH-1 governs vital transcriptional programs in endocrine sensitive and resistant breast cancer cells |
|
| Relations |
| BioProject |
PRJNA203306 |
| SRA |
SRP022857 |
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