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Series GSE47110 Query DataSets for GSE47110
Status Public on May 20, 2014
Title Illumina array to compare whole-genome expression change in miR-127 KO mice at E18.5 in 3 tissues: brain, skin and muscle
Organism Mus musculus
Experiment type Expression profiling by array
Summary miR-127 is an imprinted microRNA on mouse chromosome 12, strongly expressed during late embryogenesis and known regulator of placental gene Rtl1. miR-127-knockout (KO) mice appear phenotypically normal. An Illumina beadchip whole genome microarray experiment was carried out on embryonic stage 18.5 (E18.5) mice with a deletion in the miR-127 gene, and compared with wild type (WT) mice. Three tissues with varying expression of miR-127 were analysed: brain, skin and muscle.
 
Overall design For each tissue (brain, skin, muscle) and genotype (WT or miR-127 KO), total RNA from 15 different embryos was extracted. These RNA samples were divided into three pools of five, to make three biological replicates. Each biological replicate was applied to two separate Illumina Mouse WG-6 v2.0 beadchips, to make two technical replicates.
 
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Submission date May 20, 2013
Last update date Jan 16, 2019
Contact name Sarah E Allen
E-mail(s) sa413@cam.ac.uk
Organization name University of Cambridge
Department PDN
Lab AFS
Street address Downing Street
City Cambridge
ZIP/Postal code CB2 3EG
Country United Kingdom
 
Platforms (1)
GPL6887 Illumina MouseWG-6 v2.0 expression beadchip
Samples (36)
GSM1145078 KO Skin biological replicate 1 technical replicate 1
GSM1145079 KO Skin biological replicate 1 technical replicate 2
GSM1145080 WT Skin biological replicate 3 technical replicate 1
Relations
BioProject PRJNA203647

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE47110_RAW.tar 15.8 Mb (http)(custom) TAR
GSE47110_supplementaryData.Rdata.gz 51.4 Mb (ftp)(http) RDATA
Processed data included within Sample table

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