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| Status |
Public on Nov 04, 2013 |
| Title |
Effect of propranolol on differential gene expression in peripheral blood monocytes from mice subject to repeated social defeat |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
This study tested the effects of repeated social defeat (RSD) on gene expression in peripheral blood monocytes and examined the extent to which these effects were abrogated by the beta-adrenergic antagonist propranolol.
Study Type: Risk prediction
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| Overall design |
Gene expression profiling was carried out on peripheral blood monocyte mRNA samples collected from 36 mice randomized to either 6 cycles of repeated social defeat (RSD, n=18) or to parallel home cage control (HCC, n=18) conditions. Within each condition (RSD vs HCC), 9 animals were treated with the beta-adrenergic antagonist propranolol and 9 were treated with an equivalent volume of vehicle. After 6 cycles of RSD or parallel HCC, blood samples were pooled into groups of n=3 samples in each condition, and Illumina Mouse Ref-8 BeadArray assays were performed on RNA from approximately 1 million CD11b+ peripheral blood mononuclear cells (i.e., monocytes) which were immunomagnetically isolated by MACS. The primary research questions are 1) whether expression of pro-inflammatory genes is altered by RSD, and 2) whether treatment with propranolol abrogates these effects.
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| Contributor(s) |
Cole S |
| Citation(s) |
24062448 |
| Submission date |
May 21, 2013 |
| Last update date |
Jun 14, 2018 |
| Contact name |
Steve Cole |
| Organization name |
UCLA School of Medicine
|
| Department |
Medicine
|
| Street address |
11-934 Factor Bldg, UCLA
|
| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90095-1678 |
| Country |
USA |
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| Platforms (1) |
| GPL6885 |
Illumina MouseRef-8 v2.0 expression beadchip |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA204592 |
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