 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Oct 09, 2013 |
| Title |
ChIP-seq of MYST acetyltransferases |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Histone acetyltransferases (HAT) assemble into multisubunit complexes in order to target distinct lysine residues on nucleosomal histones. Here, we characterize native HAT complexes assembled by the BRPF-family of scaffold proteins. Their PHD-ZnKnuckle-PHD domain is essential for binding chromatin and restricted to unmethylated H3K4, a specificity that is reversed by the associated ING subunit. Native BRPF1 complexes can contain either MOZ/MORF or HBO1 as catalytic acetyltransferase subunit. Interestingly, while the previously reported HBO1 complexes containing JADE scaffold proteins target histone H4, the HBO1-BRPF1 complex acetylates only H3 in chromatin. We mapped a small region at the N-terminus of scaffold proteins responsible for histone tail selection on chromatin. Thus, alternate choice of subunits associated with HBO1 can switch its specificity from H4 to H3 tails, highlighting a crucial new role of associated subunits within HAT complexes, previously thought to be intrinsic to the catalytic subunit.
|
| |
|
| Overall design |
Genome-wide mapping of MYST acetyltransferases subunits and H3K4me3 histone mark in RKO cells.
|
| |
|
| Contributor(s) |
Lalonde M, Paquet ER, Côté J |
| Citation(s) |
24065767 |
| Submission date |
May 22, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
Marie-Eve Lalonde |
| Organization name |
CHUQ
|
| Street address |
9 McMahon street
|
| City |
Quebec city |
| State/province |
Qc |
| ZIP/Postal code |
G1R 2J6 |
| Country |
Canada |
| |
|
| Platforms (2) |
| GPL9052 |
Illumina Genome Analyzer (Homo sapiens) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
|
| Samples (6)
|
|
| Relations |
| BioProject |
PRJNA205078 |
| SRA |
SRP023013 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE47190_RAW.tar |
3.2 Gb |
(http)(custom) |
TAR (of BED, BW) |
SRA Run Selector |
| Processed data provided as supplementary file |
| Raw data are available in SRA |
|
|
|
|
 |
Less...
More...