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| Status |
Public on May 30, 2013 |
| Title |
The differentially expressed genes identified in oncospheres derived from CRC108 and its metastatic variants. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Migrating cancer stem cells (MCSCs) are believed to be tumorigenic initiators of metastasis. However, the relevance of MCSCs for organ-specific metastasis in colorectal cancer remains unclear. Using in vivo selection, transcriptomic analysis and functional verification, we cultured colorectal cancer stem cells and discovered specific cell surface markers of MCSCs exhibiting distinct abilities to metastasize to the liver and lung. These results have important clinical implications as selection criterion for post-operative adjuvant therapy.
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| Overall design |
The human primary colorectal cancer cells from colorectal cancer patient (CRC108) were orthotopically injected into NOG mice. The cancer cells in the liver or lung metastases were orthotopically injected into a new set of mice. After six selection cycles, we obtained cancer cells that produce spontaneous liver (CRC108LM) or lung metastases (CRC108PM). Under serum-free conditions, all individual cells produced nonadherent, multicellular oncospheres. Total RNA extracted from oncospheres of organ-specific metastasizing variants and their parental cells was used to generate biotinylated complementary RNA (cRNA) by following the standard Agilent GeneChip protocol.
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| Contributor(s) |
Li Q |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
May 29, 2013 |
| Last update date |
Jan 23, 2019 |
| Contact name |
Li Qingquan |
| E-mail(s) |
061101040@fudan.edu.cn
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| Organization name |
fudan university
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| Street address |
No.138，Yixueyuan Rd.
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| City |
Shanghai |
| ZIP/Postal code |
200032 |
| Country |
China |
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| Platforms (1) |
| GPL6480 |
Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Probe Name version) |
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| Samples (9)
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| GSM1150677 |
CRC108 liver metastatic variant, rep#1 |
| GSM1150678 |
CRC108 liver metastatic variant, rep#2 |
| GSM1150679 |
CRC108 liver metastatic variant, rep#3 |
| GSM1150680 |
CRC108 pulmonary metastatic variant, rep#1 |
| GSM1150681 |
CRC108 pulmonary metastatic variant, rep#2 |
| GSM1150682 |
CRC108 pulmonary metastatic variant, rep#3 |
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| This SubSeries is part of SuperSeries: |
| GSE47470 |
The differentially expressed genes identified in oncospheres derived from CRC cells and its metastatic variants |
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| Relations |
| BioProject |
PRJNA205828 |
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