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| Status |
Public on Nov 09, 2016 |
| Title |
RNA immunoprecipitation (RIP) of IGF2BP3 from pancreatic cancer cell extracts cultured on fibronectin |
| Organism |
Homo sapiens |
| Experiment type |
Other
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| Summary |
IGF2BP3-bound transcripts were identified by RNA immunoprecipitation and sequencing (RIP-Seq) and bioinformatics analysis. A pancreatic cancer cell line S2-013 was cultured on fibronectin. We performed RIP of IGF2BP3 using anti-IGF2BP3 antibody from the fibronectin-stimulated S2-013 extracts, and identified the associated mRNAs using a next generation sequencer.
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| Overall design |
S2-013 cells were seeded on fibronectin and incubated for 5 h. After washing twice with PBS, cells were lysed in NP2 buffer. Lysates were immunoprecipitated with anti-IGF2BP3 antibody or with normal rabbit IgG and Dynabeads Protein G. The immunoprecipitation products were processed by Hokkaido System Science (Sapporo, Japan) using an Illumina/Solexa instrument following standard procedures.
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| Contributor(s) |
Taniuchi K |
| Citation(s) |
25216519 |
| Submission date |
Jun 03, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
Keisuke Taniuchi |
| E-mail(s) |
ktaniuchi@kochi-u.ac.jp
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| Organization name |
Kochi Medical School
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| Street address |
Kohasu
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| City |
Nankoku |
| ZIP/Postal code |
783-8505 |
| Country |
Japan |
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| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
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| Samples (2) |
| GSM1152971 |
Immunoprecipitated mRNAs with anti-IGF2BP3 antibody |
| GSM1152972 |
Immunoprecipitated mRNAs with control IgG |
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| Relations |
| BioProject |
PRJNA206465 |
| SRA |
SRP023532 |
