|
| Status |
Public on Mar 02, 2017 |
| Title |
Differentiation of human and murine induced pluripotent stem cells to microglia-like cells [mouse] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Microglia are the resident inflammatory cells of the central nervous system (CNS) and have important roles in development, homeostasis and a variety of neurologic and psychiatric diseases. Difficulties in procuring human microglia have limited their study and hampered the clinical translation of microglia-based treatments shown to be effective in animal disease models. Here, we report the differentiation of human induced pluripotent stem cells (iPSC) into microglia-like cells by exposure to defined factors and co-culture with astrocytes. These iPSC-derived microglia (iPS-MG) have the phenotype, gene expression profile and functional properties of brain-isolated microglia. Murine iPS-MG generated using a similar protocol have equivalent efficacy to primary brain-isolated microglia in the treatment of murine syngeneic intracranial malignant gliomas. The ability to generate human microglia facilitates the further study of this important CNS cell type and raises the possibility of their use in personalized medicine applications.
|
| |
|
| Overall design |
24 Mouse samples: 3 iPSC (induced pluripotent stem cell), 3 iPS-MG (GFP+ iPSC-derived microglia), 3 neo-MG (neonatal microglia), 3 ad-MG (adult microglia), 3 DC (dendritic cell), 3 NSC (neural stem cell), 3 bm-Mac (bone marrow macrophage), 3 p-Mac (peritoneal macrophage).
|
| |
|
| Contributor(s) |
Pandya H, Shen MJ, Ichikawa DM, Sedlock AB, Choi Y, Johnson KR, Kim G, Brown M, Elkhaloun A, Maric D, McGavern DB, Malech HL, Park JK |
| Citation(s) |
28253233 |
| Submission date |
Jun 03, 2013 |
| Last update date |
Apr 18, 2017 |
| Contact name |
Kory R Johnson |
| E-mail(s) |
johnsonko@ninds.nih.gov
|
| Phone |
301-402-1956
|
| Organization name |
NINDS/NIH
|
| Department |
DIR IT & Bioinformatics
|
| Lab |
Bioinformatics Section
|
| Street address |
10/3B01, 9000 Rockville Pike
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL11533 |
[MoGene-1_1-st] Affymetrix Mouse Gene 1.1 ST Array [transcript (gene) version] |
|
| Samples (24)
|
|
| This SubSeries is part of SuperSeries: |
| GSE78116 |
Differentiation of human and murine induced pluripotent stem cells to microglia-like cells |
|
| Relations |
| BioProject |
PRJNA206513 |
Less...
More...