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| Status |
Public on Dec 31, 2013 |
| Title |
Nuclear phosphatidylinositol-5-phosphate regulates ING2 stability at discrete chromatin targets in response to DNA damage [expression] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
ING2 (inhibitor of growth family member 2) is a component of a chromatin-regulatory complex that represses gene expression and is implicated in cellular processes that promote tumor suppression. However, few direct genomic targets of ING2 have been identified and the mechanism(s) by which ING2 selectively regulates genes remains unknown. Here we provide evidence that direct association of ING2 with the nuclear phosphoinositide phosphatidylinositol-5-phosphate (PtdIns(5)P) regulates a subset of ING2 targets in response to DNA damage. At these target genes, the binding event between ING2 and PtdIns(5)P is required for ING2 promoter occupancy and ING2-associated gene repression. Moreover, depletion of PtdIns(5)P attenuates ING2-mediated regulation of these targets in the presence of DNA damage. Taken together, these findings support a model in which PtdIns(5)P functions as a sub-nuclear trafficking factor that stabilizes ING2 at discrete genomic sites.
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| Overall design |
Genome-wide expression profiling of HT1080 cells stably transduced with ING2 or a ING2 lipid binding mutant in the presence of vehicle (DMSO) or etoposide. Each condition is tested in triplicate
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| Contributor(s) |
Bua DJ, Mas Martin G, Binda O, Gozani O |
| Citation(s) |
23823870 |
| Submission date |
Jun 05, 2013 |
| Last update date |
Aug 16, 2018 |
| Contact name |
Dennis Jacob Bua |
| E-mail(s) |
dbua@stanford.edu
|
| Organization name |
Stanford University
|
| Department |
Biology
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| Street address |
Gilbert Building, Room 109, 371 Serra Mall
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| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305-5020 |
| Country |
USA |
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| Platforms (1) |
| GPL6947 |
Illumina HumanHT-12 V3.0 expression beadchip |
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| Samples (12)
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| This SubSeries is part of SuperSeries: |
| GSE47689 |
Nuclear phosphatidylinositol-5-phosphate regulates ING2 stability at discrete chromatin targets in response to DNA damage |
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| Relations |
| BioProject |
PRJNA207300 |
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