|
| Status |
Public on Jun 01, 2015 |
| Title |
Genomic instability of human embryonic stem cells in mechanic and enzymatic passaging culture |
| Organism |
Homo sapiens |
| Experiment type |
Genome variation profiling by SNP array
|
| Summary |
hESC have morphologic, genetic and genomic alternatiions when cells cultured in different passaging condition.
Here sub-karyotypic genome change in three different hESC lines were compared in two passaging methods.
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| Overall design |
Cells had initially mechanic passaging. From the initiating passage (p13 for HD291, p16 for HD129 and p25 for HS306), cell were switched to enzymatic passaging. At indicated passges, the sub-karyotypic changes in genome were analysed by Affymetrix SNP6.0 microarray.
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| |
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| Contributor(s) |
BAI Q, DE VOS J |
| Citation(s) |
25254421 |
| Submission date |
Jun 13, 2013 |
| Last update date |
Sep 20, 2021 |
| Contact name |
Qiang BAI |
| E-mail(s) |
qiang.bai@uliege.be
|
| Phone |
+32 (0)4 366 9841
|
| Organization name |
University of Liege
|
| Department |
GIGA Institute
|
| Lab |
Immunophysiology Laboratory
|
| Street address |
Avenue de l'Hopital 1
|
| City |
Liège |
| ZIP/Postal code |
4000 |
| Country |
Belgium |
| |
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| Platforms (1) |
| GPL6801 |
[GenomeWideSNP_6] Affymetrix Genome-Wide Human SNP 6.0 Array |
|
| Samples (19)
|
| GSM1162366 |
HD129 line cells at initial passage 16 |
| GSM1162367 |
HD129 line cells after 10 enzymatic passages |
| GSM1162368 |
HD129 line cells after 5 enzymatic passages |
| GSM1162370 |
HD129 line cells after 12 mechanic passages |
| GSM1162371 |
HD129 line cells after 5 mechanic passages |
| GSM1162372 |
HD291 line cells at initial passage 13 |
| GSM1162373 |
HD291 line cells after 15 enzymatic passages |
| GSM1162374 |
HD291 line cells after 30 enzymatic passages |
| GSM1162376 |
HD291 line cells after 30 mechanic passages |
| GSM1162377 |
HD291 line cells after 30 enzymatic and 15 mechanic passages |
| GSM1162378 |
HD291 line cells after 30 mechanic and 15 enzymatic passages |
| GSM1162379 |
HD291 line cells after 15 mechanic passages |
| GSM1162383 |
HS306 line cells at initial passage 25 |
| GSM1162384 |
HS306 line cells after 10 enzymatic passages |
| GSM1162385 |
HS306 line cells after 5 enzymatic passages |
| GSM1162386 |
HS306 line cells after 10 mechanic passages |
| GSM1162387 |
HS306 line cells after 7 mechanic passages |
| GSM1162388 |
HD129 line cells after 9 mechanic passages in presence of Y27632 |
| GSM1162389 |
HS306 line cells after 9 mechanic passages in presence of Y27632 |
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| This SubSeries is part of SuperSeries: |
| GSE47917 |
Genome alterations in human pluripotent stem cells as very early events highly dependent on cell passaging conditions |
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| Relations |
| BioProject |
PRJNA208331 |
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