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Series GSE47916 Query DataSets for GSE47916
Status Public on Jun 01, 2015
Title Expression of four hESC lines in enzymatic and mechanic passaging methods
Organism Homo sapiens
Experiment type Expression profiling by array
Summary hESC have morphologic, genetic and genomic alternatiions when cells cultured in different passaging condition.
Here transcriptome of four different hESC lines were compared in two passaging methods.
 
Overall design Cells had initially mechanic passaging. From the initiating passage (p13 for HD291, p16 for HD129 and p25 for HS306), cell were switched to enzymatic passaging. At indicated passges, the transcriptome were analysed by microarray.
 
Contributor(s) BAI Q, DE VOS J
Citation(s) 25254421
Submission date Jun 13, 2013
Last update date Sep 20, 2021
Contact name Qiang BAI
E-mail(s) qiang.bai@uliege.be
Phone +32 (0)4 366 9841
Organization name University of Liege
Department GIGA Institute
Lab Immunophysiology Laboratory
Street address Avenue de l'Hopital 1
City Liège
ZIP/Postal code 4000
Country Belgium
 
Platforms (1)
GPL13667 [HG-U219] Affymetrix Human Genome U219 Array
Samples (8)
GSM1162390 HD129 line after 11 enzymatic passages
GSM1162391 HD129 line after 10 mechanic passages
GSM1162392 HD291 line after 15 enzymatic passages
This SubSeries is part of SuperSeries:
GSE47917 Genome alterations in human pluripotent stem cells as very early events highly dependent on cell passaging conditions
Relations
BioProject PRJNA208330

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE47916_RAW.tar 20.5 Mb (http)(custom) TAR (of CEL, CHP)
Processed data included within Sample table
Processed data provided as supplementary file

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