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| Status |
Public on Jun 01, 2015 |
| Title |
Transcriptome of embryonic stem cells cultured in enzymatic and mechanic passaging methods [RNA-seq] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
hESC have morphologic, genetic and genomic alternatiions when cells cultured in different passaging condition.
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| Overall design |
Cells had initially mechanic passaging. From the initiating passage p13, cell were switched to enzymatic passaging after 15 passage (p13+EP15). Another sample was from cells after 15 mechanic passages (p13+MP15) which was designed as control. The transcripome of p13, p13+EP15 and p13+MP15 was analyzed with RNA sequencing by Illumina HiSeq2000 next-generation sequencer.
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| |
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| Contributor(s) |
BAI Q, DE VOS J |
| Citation(s) |
25254421 |
| Submission date |
Jun 14, 2013 |
| Last update date |
Sep 20, 2021 |
| Contact name |
Qiang BAI |
| E-mail(s) |
qiang.bai@uliege.be
|
| Phone |
+32 (0)4 366 9841
|
| Organization name |
University of Liege
|
| Department |
GIGA Institute
|
| Lab |
Immunophysiology Laboratory
|
| Street address |
Avenue de l'Hopital 1
|
| City |
Liège |
| ZIP/Postal code |
4000 |
| Country |
Belgium |
| |
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| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
|
| Samples (3) |
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| This SubSeries is part of SuperSeries: |
| GSE47917 |
Genome alterations in human pluripotent stem cells as very early events highly dependent on cell passaging conditions |
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| Relations |
| BioProject |
PRJNA208433 |
| SRA |
SRP026033 |
