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| Status |
Public on Apr 03, 2015 |
| Title |
MAQC-III/SEQC Main Study ABCD titration experiment, ERCC spike-in profiles and gene-level estimates for a selected test set |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Comparison to RNA-Seq showed different strengths and weaknesses for different regimes of expression strength. At sufficient read-depth, both platforms seemed comparable for gene-level expression profiling.
Comparison to RNA-Seq showed comparable accuracy and precision with microarrays for ERCC spike-ins at medium to high expression levels. At low expression levels, the array showed signal attenuation but better precision, while RNA-Seq maintained accuracy albeit with highly inflated variance. In summary, both platforms can be meaningfully applied in a similar range of expression strength.
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| Overall design |
Assessment of (precision, accuracy, reproducibility, mutual information, titration order consistency, and known mixing ratio recovery) by measurement of known-ratio mixtures of two RNA reference samples. The array probes 776 complex genes representative of the AceView gene model annotation, as well as 92 ERCC spike-in controls.
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| Contributor(s) |
Stralis-Pavese N, Łabaj PP, Clevert D, Fasold M, Hochreiter S, Binder H, Kreil DP |
| Citation(s) |
25150839, 25150838, 25254650 |
| Submission date |
Jun 17, 2013 |
| Last update date |
May 22, 2015 |
| Contact name |
Pawel P. Labaj |
| Organization name |
Boku University Vienna
|
| Department |
Department of Biotechnology
|
| Lab |
Chair of Bioinformatics
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| Street address |
Muthgasse 18
|
| City |
Vienna |
| ZIP/Postal code |
1190 |
| Country |
Austria |
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| Platforms (1) |
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| Samples (16)
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| This SubSeries is part of SuperSeries: |
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| Relations |
| BioProject |
PRJNA208822 |
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