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| Status |
Public on Jul 01, 2013 |
| Title |
Regulation of transcription by MLL2 complex and MLL complexes-associated protein AKAP95 |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Although histone H3 lysine 4 (H3K4) methylation is widely associated with gene activation, direct evidence for its causal role in transcription, through specific MLL family members, is scarce. Here we have purified a human MLL2 (Kmt2b) complex that is highly active in H3K4 methylation and chromatin transcription in a cell-free system. This effect requires SAM and intact H3K4, establishing a direct and causal role for MLL2-mediated H3K4 methylation in transcription. We then show that human AKAP95, a chromatin-associated protein, is physically and functionally associated with the Dpy-30-MLL complexes and directly enhances their methyltransferase activity. Ectopic AKAP95 stimulates expression of a chromosomal reporter in synergy with MLL1 or MLL2, whereas AKAP95 depletion impairs retinoic acid-mediated gene induction in embryonic stem cells. These results demonstrate an important role for AKAP95 in regulating histone methylation and gene expression, particularly during cell fate transitions.
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| Overall design |
Total RNAs from control or knockdown cells before and after RA-mediated differentiation were subjected to Illumina microarray analyses.
The complete dataset containing non_normalized, median-normalized and expression ratio (relative to Scramble shRNA, undifferentiated data) is linked below as a supplementary file [complete_data.txt].
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| |
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| Contributor(s) |
Jiang H, Roeder RG |
| Citation(s) |
23995757 |
| Submission date |
Jun 19, 2013 |
| Last update date |
Jun 14, 2018 |
| Contact name |
Hao Jiang |
| E-mail(s) |
haojiang@uab.edu
|
| Organization name |
University of Alabama at Birmingham
|
| Department |
Biochemistry and Molecular Genetics
|
| Street address |
1825 University Blvd
|
| City |
Birmingham |
| State/province |
AL |
| ZIP/Postal code |
35294 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL6885 |
Illumina MouseRef-8 v2.0 expression beadchip |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA208969 |
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