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Status |
Public on Dec 25, 2013 |
Title |
ChIP-seq in MRC5 hIPSCs overexpressing MEG3 RNA. |
Organism |
Homo sapiens |
Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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Summary |
This experiment was designed to detect differences in PRC2 occupancy in a human induced pluripotent cell line (MRC5) naturally deficient in MEG3 RNA after lentiviral overexpression of MEG3.
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Overall design |
MRC5 were transduced with lentiviruses for the overexpression of MEG3 lncRNA or GFP as a control. ChIP-seq was performed with EZH2 and JARID2 antibodies.
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Contributor(s) |
Kaneko S, Bonasio R, Reinberg D |
Citation(s) |
24374312 |
Submission date |
Jul 03, 2013 |
Last update date |
Feb 04, 2022 |
Contact name |
Roberto Bonasio |
Organization name |
University of Pennsylvania
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Department |
Cell and Developmental Biology
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Lab |
Bonasio
|
Street address |
3400 Civic Center Blvd - SCTR 9-111
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City |
Philadelphia |
State/province |
PA |
ZIP/Postal code |
19104 |
Country |
USA |
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Platforms (1) |
GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
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Samples (6)
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This SubSeries is part of SuperSeries: |
GSE48518 |
Interactions between JARID2 and noncoding RNAs regulate PRC2 recruitment to chromatin |
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Relations |
BioProject |
PRJNA210418 |
SRA |
SRP026532 |