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| Status |
Public on Jul 08, 2013 |
| Title |
Genome-wide mRNA expression analysis of primary cultured mouse brain microvascular endothelial cells after inhibition of PKC-beta |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Pharmacological inhibition of protein kinase C beta (PKC-beta) # by the compound LY317615 has been tested on primary cultured mouse brain microvascular endothelial cells, stimulated in-vitro for 8h at a concentration of 5 µM. The aim of the study was the question if inhibition of PKC-beta leads to inhibition of genes responsible for T-cell migration in order to investigate LY-317615 as a possible experimental therapy for multiple sclerosis.
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| Overall design |
primary cultured mouse brain microvascular endothelial cells (MBMEC) have been prepared from new-born mice and cultured for one week. Then stimulated for 8h with 5 µM LY317615. Two samples had been treated for the last 24h with 500 µM TNF-alpha and 500 µM IFN-gamma (inflamed) whereas two samples had been left untreated (naive). RNA was prepared using TRIZOL according to standard protocols.
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| Contributor(s) |
Lanz TV, Platten M |
| Citation(s) |
23959874 |
| Submission date |
Jul 07, 2013 |
| Last update date |
Jan 16, 2019 |
| Contact name |
Tobias Volker Lanz |
| E-mail(s) |
t.lanz@dkfz.de
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| Organization name |
German Cancer Research Center (DKFZ)
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| Department |
Clinical Cooperation Unit Neuroimmunology and Brain Tumor Immunology
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| Street address |
Im Neuenheimer Feld 280
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| City |
Heidelberg |
| ZIP/Postal code |
69120 |
| Country |
Germany |
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| Platforms (1) |
| GPL6887 |
Illumina MouseWG-6 v2.0 expression beadchip |
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| Samples (12)
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| GSM1181377 |
MBMEC, no LY-317615 treatment. [Naive ctrl 1x] |
| GSM1181378 |
MBMEC, no LY-317615 treatment. [Naive ctrl 3x] |
| GSM1181379 |
MBMEC, no LY-317615 treatment. [Naive ctrl 3] |
| GSM1181380 |
MBMEC, 5 µM LY-317615 treatmen for 8h. [Naive Ly1x] |
| GSM1181381 |
MBMEC, 5 µM LY-317615 treatmen for 8h. [Naive Ly3x] |
| GSM1181382 |
MBMEC, 5 µM LY-317615 treatmen for 8h. [Naive Ly3] |
| GSM1181383 |
MBMEC, treated for 24h with 500 µM TNF-alpha and 500 µM IFN-gamma. No LY-317615 treatment. [Inflame ctrl 2x] |
| GSM1181384 |
MBMEC, treated for 24h with 500 µM TNF-alpha and 500 µM IFN-gamma. No LY-317615 treatment. [Inflame ctrl 3x] |
| GSM1181385 |
MBMEC, treated for 24h with 500 µM TNF-alpha and 500 µM IFN-gamma. No LY-317615 treatment. [Inflame ctrl 3] |
| GSM1181386 |
MBMEC, treated for 24h with 500 µM TNF-alpha and 500 µM IFN-gamma. 5 µM LY-317615 treatment for 8h. [Inflame Ly 1x] |
| GSM1181387 |
MBMEC, treated for 24h with 500 µM TNF-alpha and 500 µM IFN-gamma. 5 µM LY-317615 treatment for 8h. [Inflame Ly3x] |
| GSM1181388 |
MBMEC, treated for 24h with 500 µM TNF-alpha and 500 µM IFN-gamma. 5 µM LY-317615 treatment for 8h. [Inflame Ly3] |
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| Relations |
| BioProject |
PRJNA210721 |
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