|
| Status |
Public on Mar 28, 2014 |
| Title |
RNA-seq of Tumor Initiating Cells (TICs) from Lung Squamous Cell Carcinoma (LSCC) lines reveals PRKCI dependent Hedgehog (HH) Signalling |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
RNA-seq analysis was performed on TICs and the parental counterparts of 4 LSCC cell lines. In addition, PRKCI knock down (KD) variants of these cells were sequenced. Subsequent analysis revealed that activation of HH signaling, a known driver of the TIC phenotype, is dependent on PRKCI expression.
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| |
|
| Overall design |
Examination of TICS, parental cells, parental PRKCI KD cells, and TIC PRKCI KD cells
|
| |
|
| Contributor(s) |
Fields AP, Justilein V, Walsh MP, Thompson EA |
| Citation(s) |
24525231 |
| Submission date |
Jul 08, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
Michael Patrick Walsh |
| E-mail(s) |
walsh.michael@mayo.edu
|
| Phone |
904-953-6055
|
| Organization name |
Mayo Clinic Jacksonville
|
| Department |
Cancer Basic Science
|
| Lab |
Alan P. Fields
|
| Street address |
4500 San Pablo Road
|
| City |
Jacksonville |
| State/province |
Florida |
| ZIP/Postal code |
32224 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL9115 |
Illumina Genome Analyzer II (Homo sapiens) |
|
| Samples (12)
|
|
| Relations |
| BioProject |
PRJNA210778 |
| SRA |
SRP026620 |
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